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Ferric Chloride-Induced Arterial Thrombosis and Sample Collection for 3D Electron Microscopy Analysis

Article DOI: 10.3791/64985-v • 03:57 min • March 17th, 2023
March 17th, 2023 •
*1, 1, 1, 1, 1, 1, 2, 3, 3, 2, *1
1Department of Molecular and Cellular Biochemistry, University of Kentucky, 2Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, 3Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
* These authors contributed equally

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The present protocol describes how to use a FeCl3-mediated injury to induce arterial thrombosis, and how to collect and prepare arterial injury samples at various stages of thrombosis for electron microscopy analysis.

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Ferric Chloride Arterial Thrombosis Sample Collection 3D Electron Microscopy Analysis Platelets Hemostatic System Thrombus Growth Architecture Cellular Level Reproducible Method Surgical Area Carotid Artery Injury Site Marking Iron Chloride Injury Flow Measurements Filter Paper Saline Residual Iron Chloride Flow Reading Probe Fixative
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