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使用 体内 显微注射和电穿孔的体细胞基因组工程小鼠模型
Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
Journal (Cancer Research)
Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
Image Acquisition and Three-Dimensional Reconstruction of Mitochondrial Cristae (Video) | JoVE
Journal (Cancer Research)
Image Acquisition and Three-Dimensional Reconstruction of Mitochondrial Cristae (Video) | JoVE
 
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使用 体内 显微注射和电穿孔的体细胞基因组工程小鼠模型

Article DOI: 10.3791/65131-v • 08:06 min • May 5th, 2023
May 5th, 2023 •
1,2, 1,3, 1,2,3
1Rosalind and Morris Goodman Cancer Research Institute, McGill University, 2Department of Human Genetics, McGill University, 3McGill Integrated Core for Animal Modeling (MICAM), McGill University

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该协议描述了显微注射和 体内 电穿孔，用于小鼠输卵管中区域限制的CRISPR介导的基因组编辑。

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癌症研究，第195期，CRISPR/Cas，基因组编辑，输卵管，输卵管，卵巢癌， 体内 癌症建模
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