High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

Melissa L. Blotter; Isaac W. Stubbs; Jacob H. Norby; Maxwell Holmes; Ben Kearsley; Alexis Given; Kutter Hine; Micah R. Shepherd; R. Ryley Parrish

doi:10.3791/67065

Actividad similar a las convulsiones de alta calidad a partir de cortes agudos de cerebro utiliza...

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Neuroscience

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JoVE Journal Neuroscience

High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

Actividad similar a las convulsiones de alta calidad a partir de cortes agudos de cerebro utilizando un sistema complementario de matriz de microelectrodos de alta densidad de semiconductores de óxido metálico

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06:28 min

September 27, 2024

DOI: 10.3791/67065-v

Melissa L. Blotter*^1,2, Isaac W. Stubbs*^1,2, Jacob H. Norby*^1,2, Maxwell Holmes^1,2, Ben Kearsley³, Alexis Given¹, Kutter Hine^1,4, Micah R. Shepherd⁵, R. Ryley Parrish^1,2

¹Department of Cell Biology and Physiology,Brigham Young University, ²Neuroscience Center,Brigham Young University, ³Department of Statistics,Brigham Young University, ⁴Department of Biology,Brigham Young University, ⁵Department of Physics and Astronomy,Brigham Young University

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Overview

This study outlines a protocol for using complementary metal-oxide-semiconductor high-density microelectrode array systems (CMOS-HD-MEAs) to investigate seizure-like activity from ex vivo brain slices. The research aims to enhance the understanding of seizure initiation, propagation, and termination, with a focus on developing novel therapies for status epilepticus.

Key Study Components

Area of Science

Neuroscience
Electrophysiology
Epileptology

Background

Seizures can have complex initiation and propagation mechanisms.
Status epilepticus is a critical condition that often resists treatment.
High-density microelectrode arrays provide detailed electrophysiological data.
Different paradigms offer insights into the dynamics of seizure-like activity.

Purpose of Study

To establish a reliable protocol for recording seizure activity.
To explore the spatial and temporal patterns of status epilepticus.
To inform the development of more effective treatments for severe seizures.

Methods Used

The main platform used is CMOS-HD-MEAs with ex vivo brain slices.
Brain slices are subjected to various treatment paradigms to investigate seizure-like activity.
Detailed preparation and maintenance steps are provided for optimal recordings.
The protocol includes steps for preparing the microelectrode array and ensuring proper environmental conditions.
Electrophysiological activity is recorded to analyze seizure characteristics.

Main Results

Neocortical regions show significant electrographic seizure-like activity under specific conditions.
Hippocampal regions exhibited variability in seizure-like activity.
Different paradigms revealed distinct power dynamics in seizure frequencies.

Conclusions

This study facilitates a deeper understanding of seizure mechanisms using advanced technology.
The findings may contribute to identifying new therapeutic targets for epilepsy treatment.
The research emphasizes the importance of spatial and temporal analysis in understanding seizure activity.

Frequently Asked Questions

What are the advantages of using CMOS-HD-MEAs?

CMOS-HD-MEAs allow for high-resolution recordings of electrophysiological data, providing detailed insights into brain activity and seizure dynamics.

How is seizure activity recorded in this study?

Seizure activity is recorded from brain slices using CMOS-HD-MEAs, which capture local field potentials during various treatment paradigms.

What types of data can be obtained from the CMOS-HD-MEAs?

The technology enables the recording of high-resolution local field potentials, facilitating analysis of seizure patterns and dynamics in the brain.

How can the method be applied to other research?

This protocol can be adapted for studying various neurological disorders by altering the treatment paradigms applied to the brain slices.

Are there any limitations to using this approach?

Some limitations may include the variability between brain slices and the need for precise experimental conditions to ensure consistent results.

Aquí, describimos un protocolo para el uso de sistemas complementarios de matriz de microelectrodos de alta densidad de semiconductores de óxido metálico (CMOS-HD-MEA) para registrar la actividad similar a las convulsiones de cortes de cerebro ex vivo .

En el laboratorio de Parrish, estamos interesados en comprender cómo comienzan, se propagan y terminan las convulsiones. Estamos particularmente interesados en explorar terapias novedosas para el estado epiléptico, una afección potencialmente mortal en la que una convulsión no termina por sí sola. Utilizamos sistemas de matriz de microelectrodos de alta densidad CMOS en nuestra investigación.

Estas tecnologías avanzadas nos permiten registrar datos electrofisiológicos de alta resolución a partir de cortes de cerebro, capturando potenciales de campo locales detallados. Esto nos ayuda a comprender las actividades cerebrales complejas, como los patrones de convulsiones, con gran precisión espacial y temporal. En el futuro, planeamos explorar los patrones de propagación espacial y temporal del estado epiléptico, un estado convulsivo prolongado que a menudo se vuelve resistente a la medicación antiepiléptica.

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