Michael R. Emmert-Buck Laboratory of Pathology, National Cancer Institute National Institutes of Health Biography Publications Institution JoVE Articles Michael R. Emmert-Buck has not added a biography. If you are Michael R. Emmert-Buck and would like to personalize this page please email our Author Liaison for assistance. Publications Multiplex Quantitative Measurement of MRNAs from Fixed Tissue Microarray Sections Applied Immunohistochemistry & Molecular Morphology : AIMM / Official Publication of the Society for Applied Immunohistochemistry. May-Jun, 2014 | Pubmed ID: 24809843 Deletion of the Olfactomedin 4 Gene is Associated with Progression of Human Prostate Cancer The American Journal of Pathology. Oct, 2013 | Pubmed ID: 24070418 Validation of Esophageal Squamous Cell Carcinoma Candidate Genes from High-throughput Transcriptomic Studies American Journal of Cancer Research. 2013 | Pubmed ID: 23977449 Analysis of Transcription Factor MRNAs in Identified Oxytocin and Vasopressin Magnocellular Neurons Isolated by Laser Capture Microdissection PloS One. 2013 | Pubmed ID: 23894472 Measuring Collective Cell Movement and Extracellular Matrix Interactions Using Magnetic Resonance Imaging Scientific Reports. 2013 | Pubmed ID: 23698816 Proteomic Analysis of Frozen Tissue Samples Using Laser Capture Microdissection Methods in Molecular Biology (Clifton, N.J.). 2013 | Pubmed ID: 23625395 Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications Methods in Molecular Biology (Clifton, N.J.). 2013 | Pubmed ID: 23359150 Three-dimensional MRNA Measurements Reveal Minimal Regional Heterogeneity in Esophageal Squamous Cell Carcinoma The American Journal of Pathology. Feb, 2013 | Pubmed ID: 23219752 Semiautomated Laser Capture Microdissection of Lung Adenocarcinoma Cytology Samples Acta Cytologica. 2012 | Pubmed ID: 23207440 Integration of Architectural and Cytologic Driven Image Algorithms for Prostate Adenocarcinoma Identification Analytical Cellular Pathology (Amsterdam). 2012 | Pubmed ID: 22425661 Identification of Unique Expression Signatures and Therapeutic Targets in Esophageal Squamous Cell Carcinoma BMC Research Notes. 2012 | Pubmed ID: 22280838 Image Microarrays (IMA): Digital Pathology's Missing Tool Journal of Pathology Informatics. 2011 | Pubmed ID: 22200030 A Dynamic Magnetic Shift Method to Increase Nanoparticle Concentration in Cancer Metastases: a Feasibility Study Using Simulations on Autopsy Specimens International Journal of Nanomedicine. 2011 | Pubmed ID: 22131836 American Journal of Cancer Research: Editorial Board (2011) American Journal of Cancer Research. 2011 | Pubmed ID: 22016829 Launching of American Journal of Cancer Research American Journal of Cancer Research. 2011 | Pubmed ID: 21969259 Decrease in CD8+ Lymphocyte Number and Altered Cytokine Profile in Human Prostate Cancer American Journal of Cancer Research. 2011 | Pubmed ID: 21969236 Squamous Cell Carcinoma - Similarities and Differences Among Anatomical Sites American Journal of Cancer Research. Jan, 2011 | Pubmed ID: 21938273 MicroRNA Analysis of Microdissected Normal Squamous Esophageal Epithelium and Tumor Cells American Journal of Cancer Research. 2011 | Pubmed ID: 21796275 Immunoguided Microdissection Techniques Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21761293 Why is It Crucial to Reintegrate Pathology into Cancer Research? BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology. Jul, 2011 | Pubmed ID: 21590787 SIVQ-aided Laser Capture Microdissection: A Tool for High-throughput Expression Profiling Journal of Pathology Informatics. 2011 | Pubmed ID: 21572509 Quantifying MRNA Levels Across Tissue Sections with 2D-RT-qPCR Analytical and Bioanalytical Chemistry. Jul, 2011 | Pubmed ID: 21559756 Digital Slide Repositories for Publications: Lessons Learned from the Microarray Community The American Journal of Surgical Pathology. Jun, 2011 | Pubmed ID: 21552111 An NIH Intramural Percubator As a Model of Academic-industry Partnerships: from the Beginning of Life Through the Valley of Death Journal of Translational Medicine. 2011 | Pubmed ID: 21548992 Methylation Profiling of Mediastinal Gray Zone Lymphoma Reveals a Distinctive Signature with Elements Shared by Classical Hodgkin's Lymphoma and Primary Mediastinal Large B-cell Lymphoma Haematologica. Apr, 2011 | Pubmed ID: 21454882 Effect of Immunohistochemistry on Molecular Analysis of Tissue Samples: Implications for Microdissection Technologies The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society. Jun, 2011 | Pubmed ID: 21430260 Expression Microdissection Adapted to Commercial Laser Dissection Instruments Nature Protocols. Apr, 2011 | Pubmed ID: 21412274 Layered Electrophoretic Transfer - A Method for Pre-analytic Processing of Histological Sections Proteomics. Mar, 2011 | Pubmed ID: 21280224 Increased Matrix Metalloproteinase Activation in Esophageal Squamous Cell Carcinoma Journal of Translational Medicine. 2010 | Pubmed ID: 20920372 Proteomic Profiling of Cancer--opportunities, Challenges, and Context The Journal of Pathology. Sep, 2010 | Pubmed ID: 20623483 Influence of Hypoxia Induced by Minimally Invasive Prostatectomy on Gene Expression: Implications for Biomarker Analysis American Journal of Translational Research. 2010 | Pubmed ID: 20589162 Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens The Journal of Molecular Diagnostics : JMD. Jul, 2010 | Pubmed ID: 20413681 High Throughput Screening of Normal and Neoplastic Tissue Samples Combinatorial Chemistry & High Throughput Screening. Mar, 2010 | Pubmed ID: 20015016 2D-PCR: a Method of Mapping DNA in Tissue Sections Lab on a Chip. Dec, 2009 | Pubmed ID: 20024032 Identification of EpCAM As a Molecular Target of Prostate Cancer Stroma The American Journal of Pathology. Dec, 2009 | Pubmed ID: 19850885 Current Molecular Diagnostics of Breast Cancer and the Potential Incorporation of MicroRNA Expert Review of Molecular Diagnostics. Jul, 2009 | Pubmed ID: 19580430 Quantitative RT-PCR Gene Expression Analysis of Laser Microdissected Tissue Samples Nature Protocols. 2009 | Pubmed ID: 19478806 Large-scale Profiling of Archival Lymph Nodes Reveals Pervasive Remodeling of the Follicular Lymphoma Methylome Cancer Research. Feb, 2009 | Pubmed ID: 19155300 Quantitation of Steroid Hormones in Thin Fresh Frozen Tissue Sections Analytical Chemistry. Nov, 2008 | Pubmed ID: 18937426 Overexpression of CDC25B and LAMC2 MRNA and Protein in Esophageal Squamous Cell Carcinomas and Premalignant Lesions in Subjects from a High-risk Population in China Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology. Jun, 2008 | Pubmed ID: 18559558 Tissue Microdissection Methods in Molecular Biology (Clifton, N.J.). 2008 | Pubmed ID: 18369881 Quantitation of HER2 and Telomerase Biomarkers in Solid Tumors with IgY Antibodies and Nanocrystal Detection International Journal of Cancer. Journal International Du Cancer. May, 2008 | Pubmed ID: 18214859 Global Expression Analysis of Prostate Cancer-associated Stroma and Epithelia Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B. Dec, 2007 | Pubmed ID: 18043281 Assessment of Normalization Strategies for Quantitative RT-PCR Using Microdissected Tissue Samples Laboratory Investigation; a Journal of Technical Methods and Pathology. Sep, 2007 | Pubmed ID: 17643124 Layered Peptide Array for Multiplex Immunohistochemistry The Journal of Molecular Diagnostics : JMD. Jul, 2007 | Pubmed ID: 17591928 Layered Peptide Arrays: a Diverse Technique for Antibody Screening of Clinical Samples Annals of the New York Academy of Sciences. Mar, 2007 | Pubmed ID: 17435150 Increased Expression of VEGF121/VEGF165-189 Ratio Results in a Significant Enhancement of Human Prostate Tumor Angiogenesis International Journal of Cancer. Journal International Du Cancer. May, 2007 | Pubmed ID: 17278099 Layered Expression Scanning: Multiplex Molecular Analysis of Diverse Life Science Platforms Clinica Chimica Acta; International Journal of Clinical Chemistry. Feb, 2007 | Pubmed ID: 16996046 Genome-wide Loss of Heterozygosity and Copy Number Alteration in Esophageal Squamous Cell Carcinoma Using the Affymetrix GeneChip Mapping 10 K Array BMC Genomics. 2006 | Pubmed ID: 17134496 Tumor-associated Endothelial Cells Display GSTP1 and RARbeta2 Promoter Methylation in Human Prostate Cancer Journal of Translational Medicine. 2006 | Pubmed ID: 16512911 Gene Promoter Methylation in Prostate Tumor-associated Stromal Cells Journal of the National Cancer Institute. Feb, 2006 | Pubmed ID: 16478744 Layered Peptide Arrays: High-throughput Antibody Screening of Clinical Samples The Journal of Molecular Diagnostics : JMD. Oct, 2005 | Pubmed ID: 16237212 Molecular Alterations in Primary Prostate Cancer After Androgen Ablation Therapy Clinical Cancer Research : an Official Journal of the American Association for Cancer Research. Oct, 2005 | Pubmed ID: 16203770 Allelotyping of Esophageal Squamous-cell Carcinoma on Chromosome 13 Defines Deletions Related to Family History Genes, Chromosomes & Cancer. Nov, 2005 | Pubmed ID: 16015646 Profiling EGFR Activity in Head and Neck Squamous Cell Carcinoma by Using a Novel Layered Membrane Western Blot Technology Oral Oncology. May, 2005 | Pubmed ID: 15878755 Genome-wide Association Study in Esophageal Cancer Using GeneChip Mapping 10K Array Cancer Research. Apr, 2005 | Pubmed ID: 15805246 Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications Molecular Biotechnology. Feb, 2005 | Pubmed ID: 15699569 Histomathematical Analysis of Clinical Specimens: Challenges and Progress The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society. Feb, 2005 | Pubmed ID: 15684330 Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications Methods in Molecular Medicine. 2005 | Pubmed ID: 15542897 Novel Proteomic Approaches for Tissue Analysis Expert Review of Proteomics. Aug, 2004 | Pubmed ID: 15966813 Expression Microdissection: Operator-independent Retrieval of Cells for Molecular Profiling Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B. Dec, 2004 | Pubmed ID: 15538110 Comparison of Snap Freezing Versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens The Journal of Molecular Diagnostics : JMD. Nov, 2004 | Pubmed ID: 15507677 Comprehensive Characterization of Annexin I Alterations in Esophageal Squamous Cell Carcinoma Clinical Cancer Research : an Official Journal of the American Association for Cancer Research. Sep, 2004 | Pubmed ID: 15447985 Multimembrane Dot-blotting: a Cost-effective Tool for Proteome Analysis BioTechniques. Jun, 2004 | Pubmed ID: 15211757 Molecular Profiling of Cancer Toxicologic Pathology. Mar-Apr, 2004 | Pubmed ID: 15209405 High Frequency of CDKN2A Alterations in Esophageal Squamous Cell Carcinoma from a High-risk Chinese Population Genes, Chromosomes & Cancer. Mar, 2004 | Pubmed ID: 14732922 Evaluation of BRCA2 in the Genetic Susceptibility of Familial Esophageal Cancer Oncogene. Jan, 2004 | Pubmed ID: 14647438 Layered Expression Scanning: Multiplex Analysis of RNA and Protein Gels BioTechniques. Dec, 2003 | Pubmed ID: 14682064 Parathyroid Gland-specific Deletion of the Mouse Men1 Gene Results in Parathyroid Neoplasia and Hypercalcemic Hyperparathyroidism Cancer Research. Nov, 2003 | Pubmed ID: 14633735 Evidence for a Familial Esophageal Cancer Susceptibility Gene on Chromosome 13 Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology. Oct, 2003 | Pubmed ID: 14578153 Mitochondrial Proteome: Altered Cytochrome C Oxidase Subunit Levels in Prostate Cancer Proteomics. Sep, 2003 | Pubmed ID: 12973739 Gene Expression Analysis of Esophageal Squamous Cell Carcinoma Reveals Consistent Molecular Profiles Related to a Family History of Upper Gastrointestinal Cancer Cancer Research. Jul, 2003 | Pubmed ID: 12873975 Handling of Clinical Tissue Specimens for Molecular Profiling Studies Current Issues in Molecular Biology. Apr, 2003 | Pubmed ID: 12793526 Molecular Differentiation of High- and Moderate-grade Human Prostate Cancer by CDNA Microarray Analysis Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B. Jun, 2003 | Pubmed ID: 12766610 Approaches to Proteomic Analysis of Human Tumors Methods in Molecular Biology (Clifton, N.J.). 2003 | Pubmed ID: 12710699 Evaluation of Ethanol-fixed, Paraffin-embedded Tissues for Proteomic Applications Proteomics. Apr, 2003 | Pubmed ID: 12687609 The Granulin-epithelin Precursor/PC-cell-derived Growth Factor is a Growth Factor for Epithelial Ovarian Cancer Clinical Cancer Research : an Official Journal of the American Association for Cancer Research. Jan, 2003 | Pubmed ID: 12538450 Allelic Loss on Chromosome 13q14 and Mutation in Deleted in Cancer 1 Gene in Esophageal Squamous Cell Carcinoma Oncogene. Jan, 2003 | Pubmed ID: 12527901 Post-analysis Follow-up and Validation of Microarray Experiments Nature Genetics. Dec, 2002 | Pubmed ID: 12454646 Serum Proteomic Patterns for Detection of Prostate Cancer Journal of the National Cancer Institute. Oct, 2002 | Pubmed ID: 12381711 Alterations in Gene Expression Profiles During Prostate Cancer Progression: Functional Correlations to Tumorigenicity and Down-regulation of Selenoprotein-P in Mouse and Human Tumors Cancer Research. Sep, 2002 | Pubmed ID: 12235003 2D Differential In-gel Electrophoresis for the Identification of Esophageal Scans Cell Cancer-specific Protein Markers Molecular & Cellular Proteomics : MCP. Feb, 2002 | Pubmed ID: 12096129 The RASSF1A Tumor Suppressor Gene is Inactivated in Prostate Tumors and Suppresses Growth of Prostate Carcinoma Cells Cancer Research. Jun, 2002 | Pubmed ID: 12067994 Infrequent Mutation in the BRCA2 Gene in Esophageal Squamous Cell Carcinoma Clinical Cancer Research : an Official Journal of the American Association for Cancer Research. Apr, 2002 | Pubmed ID: 11948123 Loss of Heterozygosity on Chromosome Arms 3p and 6q in Microdissected Adenocarcinomas of the Uterine Cervix and Adenocarcinoma in Situ Cancer. Feb, 2002 | Pubmed ID: 11857315 Evaluation of Non-formalin Tissue Fixation for Molecular Profiling Studies The American Journal of Pathology. Feb, 2002 | Pubmed ID: 11839565 Proteomic Analysis of Human Prostate Cancer Molecular Carcinogenesis. Jan, 2002 | Pubmed ID: 11807953 Determination of a Minimal Deletion Interval on Chromosome Band 8p21 in Sporadic Prostate Cancer Genes, Chromosomes & Cancer. Feb, 2002 | Pubmed ID: 11793446 SIVQ-LCM Protocol for the ArcturusXT Instrument Jason D. Hipp*1, Jerome Cheng*2, Jeffrey C. Hanson*1, Avi Z. Rosenberg1, Michael R. Emmert-Buck1, Michael A. Tangrea1, Ulysses J. Balis2 1Laboratory of Pathology, National Cancer Institute, National Institutes of Health, 2Department of Pathology, University of Michigan JoVE 51662 Biologie
SIVQ-LCM Protocol for the ArcturusXT Instrument Jason D. Hipp*1, Jerome Cheng*2, Jeffrey C. Hanson*1, Avi Z. Rosenberg1, Michael R. Emmert-Buck1, Michael A. Tangrea1, Ulysses J. Balis2 1Laboratory of Pathology, National Cancer Institute, National Institutes of Health, 2Department of Pathology, University of Michigan JoVE 51662 Biologie