Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens (Video) | JoVE

Brittany Turner-Ivey; Jody Fromm Longo; Dorea P. Jenkins; Stephen T. Guest; Steven L. Carroll

doi:10.3791/65430

Genome-Scale Screening to Identify and Compare Therapeutic Targets in Tumors

August 25th, 2023

Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens (Video) | JoVE

01:41 min

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Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens (Video) | JoVE

03:15 min

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Lentiviral Infection of MPNST Cells and Genomic DNA Isolation for Genome-Scale shRNA Screens (Video) | JoVE

03:50 min

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Cytometer Assays of MPNST Cells Challenged with Therapeutic Agents

01:30 min

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Brittany Turner-Ivey¹, Jody Fromm Longo², Dorea P. Jenkins², Stephen T. Guest³, Steven L. Carroll^1,2

¹Hollings Cancer Center, Medical University of South Carolina, ²Department of Pathology and Laboratory Medicine, Medical University of South Carolina, ³Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor

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We have developed a cross-species comparative oncogenomics approach utilizing genomic analyses and functional genomic screens to identify and compare therapeutic targets in tumors arising in genetically engineered mouse models and the corresponding human tumor type.

Article

Genome-Scale Screening to Identify and Compare Therapeutic Targets in Tumors

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