Cyclodextrin Mediated Exchange
Cyclodextrin Mediated Exchange - Related Videos
08:21
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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Cited by 11 •
2012
A quick and efficient method to integrate foreign DNA of interest into pre-made acceptor strains, termed landing pad strains, is described. The method allows site-specific integration of a DNA cassette into the engineered landing pad locus of a given strain, through conjugation and expression of the ΦC31 integrase.
10:10
Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study
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Cited by 11 •
2016
The motion regimes of ibuprofen encapsulated in β-cyclodextrin nanosponges polymer network are investigated using pulsed-field-gradient spin-echo (PGSE) NMR technique. Synthesis, purification, drug loading, implementation of the NMR pulse sequence and data analysis to work out the mean square displacement of the drug at several observation times are described in detail.
15:13
Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
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Cited by 6 •
2017
Proteins often contain multiple domains that can exert different cellular functions. Gene knock-outs (KO) do not consider this functional diversity. Here, we report a recombination-mediated cassette exchange (RMCE)-based structure-function approach in KO embryonic stem cells that allows for the molecular dissection of various functional domains or variants of a protein.
11:38
Heat Exchanger Analysis
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2023
Source: Alexander S Rattner and Christopher J Greer; Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA Heat exchangers transfer thermal energy between two fluid streams, and are ubiquitous in energy systems. Common applications include car radiators (heat transfer from hot engine coolant to surrounding air), refrigerator evaporators (air inside refrigerator compartment to evaporating refrigerant), and cooling towers in power plants...
11:36
Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
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Cited by 3 •
2016
Here we report a rapid and efficient gene editing method based on RMCE in the AAVS1 locus of human Pluripotent Stem Cells (hPSCs) that improves upon previously described systems. Using this technique, isogenic lines can be rapidly and reliably generated for proper comparative studies, facilitating transgenesis-mediated research with hPSCs.
FAQs
The carrier combines a hydrophobic cavity with a hydrophilic exterior. The cavity temporarily accommodates a lipid molecule, while the outer surface supports movement through the surrounding aqueous solution. This arrangement separates lipid carriage from direct membrane contact, allowing lipids to be transferred between donor and acceptor membranes during a controlled biochemical experiment.
Exchange is influenced by the relative lipid concentrations in the donor and acceptor membranes. A concentration difference creates the basis for lipid movement through the cyclodextrin carrier, shifting membrane composition as transfer proceeds. Researchers can use this principle to investigate how changing lipid distribution affects membrane behavior without treating all membrane components as permanently fixed.
Selective lipid transfer lets researchers alter membrane composition while examining the resulting biochemical response. In particular, changing cholesterol distribution or lipid asymmetry can reveal how membrane properties influence protein activity and cell signaling. The approach therefore connects a defined change in membrane lipids with downstream functional observations in biochemical and biophysical systems.
Mixing membrane preparations does not by itself provide the same controlled basis for changing lipid composition between defined donor and acceptor systems. Cyclodextrin-mediated exchange uses a carrier and concentration differences to promote lipid movement, making it possible to manipulate selected membrane features and then examine how those changes influence membrane properties or associated proteins.
A general workflow begins by preparing donor and acceptor membranes or membrane models with the lipid compositions relevant to the experiment. Cyclodextrin is then used to support lipid movement between them, and the resulting membrane changes are examined. The design should preserve a clear distinction between the original donor and acceptor systems so composition changes can be interpreted.
Researchers would choose the method when they need to selectively modify membrane lipids rather than study an unchanged membrane mixture. It is especially useful for examining cholesterol distribution, lipid asymmetry, membrane properties, protein activity, or cell signaling. Because the lipid composition can be manipulated experimentally, the method supports comparisons between differently prepared membrane states.
The method can reveal how a deliberate change in membrane lipid composition affects the physical or biochemical behavior of a membrane system. Measurements or observations may focus on altered cholesterol distribution, lipid asymmetry, membrane properties, protein activity, or signaling responses. These outcomes help connect membrane composition with function in biochemical and biophysical research.