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JoVE Journal
Bioengineering

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Site-specific Bacterial Chromosome Engineering
 

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Article doi: 10.3791/3698
March 16th, 2012

Summary March 16th, 2012

A quick and efficient method to integrate foreign DNA of interest into pre-made acceptor strains, termed landing pad strains, is described. The method allows site-specific integration of a DNA cassette into the engineered landing pad locus of a given strain, through conjugation and expression of the ΦC31 integrase.

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