Ph Sensitive Fluorescence
Ph Sensitive Fluorescence - Related Videos
08:27
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
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2016
This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.
08:31
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
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Cited by 2 •
2021
This protocol presents a fluorescence imaging method that uses a class of pH-sensitive lipid fluorophores to monitor lipid membrane trafficking during cell exocytosis and the endocytosis cycle.
09:13
Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
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Cited by 33 •
2018
Mitophagy, the selective degradation of mitochondria, has been implicated in mitochondrial homeostasis and is deregulated in various human diseases. However, convenient experimental methods for measuring mitophagy activity are very limited. Here, we provide a sensitive assay for measuring mitophagy activity using flow cytometry.
08:28
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
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Cited by 3 •
2018
Here, we present a protocol for the simultaneous use of Förster resonance energy transfer-based tension sensors to measure protein load and fluorescence recovery after photobleaching to measure protein dynamics enabling the measurement of force-sensitive protein dynamics within living cells.
10:30
Dye-sensitized Solar Cells
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University Today's modern world requires the use of a large amount of energy. While we harness energy from fossil fuels such as coal and oil, these sources are nonrenewable and thus the supply is limited. To maintain our global lifestyle, we must extract energy from renewable sources. The most promising renewable source, in terms of abundance, is the sun, which provides us with more than enough solar energy to fully fuel our...
FAQs
Proton concentration changes the fluorophore’s protonation state, which in turn changes its electronic structure. Those molecular changes can alter how strongly the probe fluoresces, shift the emission wavelength, or change the relationship between signals at two wavelengths. The selected optical response determines what pH-related measurement is extracted, while the underlying mechanism remains the probe’s sensitivity to acidity or alkalinity.
Signal choice affects how pH information is interpreted. A measurement based on fluorescence intensity tracks changes in emission strength, whereas wavelength-based or ratiometric measurements compare optical signals across wavelengths. These alternatives provide different ways to represent proton conditions, so researchers must connect the chosen signal to pH through calibration rather than treating every fluorescence change as a direct pH value.
Unlike a conventional electrode, a fluorescent probe can report pH in a local region, including within a cell or subcellular compartment. This makes the optical readout useful when inserting or positioning an electrode would be impractical. In biochemical experiments, spatially resolved signals can reveal differences in proton conditions between locations rather than only a bulk solution value.
Quantitative use requires calibration: the measured fluorescence response must be related to pH so that an optical signal can be interpreted numerically. Calibration is especially important when intensity, emission wavelength, or a ratio of wavelengths serves as the readout, because each measurement format expresses proton conditions differently. Once established, the relationship supports comparisons of pH across biochemical samples.
Changes in fluorescence can be used to examine enzyme activity, membrane transport, organelle function, and biochemical reactions when those processes alter local proton conditions. The probe therefore links an optical signal to a biochemical event without reducing the analysis to a single bulk measurement. In cells, this supports investigation of how proton conditions vary across compartments or change during activity.
A calibrated fluorescence measurement can provide both pH information and evidence of proton dynamics, depending on whether the experiment compares locations, conditions, or observations over time. The resulting signal is most useful when interpreted through the established pH relationship rather than as fluorescence alone. This approach supports sensitive, spatially resolved analysis of proton behavior in solutions, cells, and subcellular compartments.