Antibody Self-association
Antibody Self-association - Related Videos
10:10
The Implicit Association Test
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2023
Source: Julian Wills & Jay Van Bavel—New York University One of the core constructs in social psychology is the notion of an attitude toward an object or person. Traditionally, psychologists measured attitudes by simply asking people to self-report their beliefs, opinions, or feelings. This approach has limitations, however, when measuring socially sensitive attitudes, like racial prejudice, because people are often motivated to self-report unprejudiced, egalitarian beliefs (despite...
13:21
Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas
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2023
Source: Frances V. Sjaastad1,2, Whitney Swanson2,3, and Thomas S. Griffith1,2,3,4 1 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Department of Urology, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Polyclonal antibodies are defined as a collection of antibodies directed against...
01:10
Antibody Structure
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2019
Overview Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding. The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains Antibodies consist of four polypeptide chains: two identical heavy...
12:55
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Cited by 33 •
2015
A method is described with visual accompaniment for conducting scalable, high throughput selections from phage-displayed combinatorial synthetic antibody libraries against hundreds of antigens simultaneously. Using this parallel approach, we have isolated antibody fragments that exhibit high affinity and specificity for diverse antigens that are functional in standard immunoassays.
01:27
Associative Learning
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2024
Associative learning is a fundamental concept in behavioral psychology, wherein a connection is established between two stimuli or events, leading to a learned response. This process is critical in understanding how behaviors are acquired and modified. Conditioning, the mechanism through which associations are formed, can be divided into two main types: classical conditioning and operant conditioning, each elucidating different aspects of associative learning. Classical conditioning, also known...
FAQs
Complementary surface regions bring antibody molecules together through intermolecular noncovalent contacts. Charged regions can interact with oppositely compatible surfaces, while hydrophobic or other matching areas can also contribute. Because these contacts are noncovalent, their combined strength can vary with solution conditions, producing different extents of association rather than a single fixed state.
Concentration, pH, ionic strength, and temperature can change how extensively antibody molecules associate. These variables should therefore be considered together when examining solution behavior, especially for concentrated preparations. Comparing association under controlled changes in each condition helps identify formulation environments that limit undesirable intermolecular interactions while preserving solution stability.
Transient clusters reflect reversible association, whereas irreversible aggregation represents a less readily reversible change in the antibody population. This distinction matters because the two behaviors have different implications for stability and formulation. Analysis that separates reversible clustering from irreversible aggregation can show whether an observed association is condition-dependent or signals a more persistent product problem.
Sequence and structural features determine which surface regions are available to form intermolecular contacts. Changes in these features can alter the balance of charged, hydrophobic, or other interactions between antibody molecules, changing their association behavior. Evaluating this relationship helps connect molecular design with solution properties such as solubility, viscosity, and stability.
A useful comparison varies antibody concentration, pH, ionic strength, and temperature while monitoring the resulting extent of association. The goal is not simply to measure one sample, but to determine how association responds to controlled changes in its environment. This approach helps identify conditions associated with transient clustering, greater viscosity, or improved solution stability.
At high antibody concentrations, intermolecular association can contribute to increased solution viscosity and reduced solubility. Studying this behavior helps guide formulation choices intended to keep the product manageable and stable. The resulting information supports development of concentrated medicines by identifying conditions or molecular features associated with less undesirable association.
Association behavior can influence decisions about purification conditions and storage formulations because solution conditions affect the extent of intermolecular contacts. Characterizing that response helps distinguish environments that favor reversible clustering from those linked with persistent aggregation. This information supports more stable handling and storage of antibody products while maintaining attention to their biophysical behavior.