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Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
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Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

DOI:
10.3791/58099-v

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09:13 min

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August 12, 2018

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, , ,
1Peripheral Neuropathy Research Center, College of Medicine,Dong-A University, 2Department of Biochemistry, College of Medicine,Dong-A University, 3Aging Institute, Department of Medicine,University of Pittsburgh School of Medicine

Chapters

  • 00:00Title
  • 01:02Generation of Cells Expressing mt-Keima
  • 03:25Inducing Mitophagy using CCCP Treatment and Preparing FACS Samples
  • 04:29FACS Analysis of Mitophagy
  • 07:16Results: Analysis of CCCP-induced Mitophagy in HeLa-mt-Keima Cells by Flow Cytometry
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

Mitophagy, the selective degradation of mitochondria, has been implicated in mitochondrial homeostasis and is deregulated in various human diseases. However, convenient experimental methods for measuring mitophagy activity are very limited. Here, we provide a sensitive assay for measuring mitophagy activity using flow cytometry.

Tags

Keywords: MitophagyFlow CytometryMt-KeimaCCCPHeLa CellsMitochondriaLentivirusPuromycin

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