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09:13 min
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August 12, 2018
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Summary
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Mitophagy, the selective degradation of mitochondria, has been implicated in mitochondrial homeostasis and is deregulated in various human diseases. However, convenient experimental methods for measuring mitophagy activity are very limited. Here, we provide a sensitive assay for measuring mitophagy activity using flow cytometry.
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Cite this Article
Um, J., Kim, Y. Y., Finkel, T., Yun, J. Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima. J. Vis. Exp. (138), e58099, doi:10.3791/58099 (2018).
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