Blood Borne Macrophages
Blood Borne Macrophages - Related Videos
18:46
Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs
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Cited by 20 •
2010
Nanoparticles of indinavir, ritonavir, efavirenz and atazanavir were manufactured using wet milling, homogenization and ultrasonication. These nanoformulations, collectively termed nanoformulated antiretroviral therapy (nanoART), assessed macrophage-based drug delivery. Monocyte-derived macrophage nanoART uptake, retention and sustained release were determined. These preliminary studies suggest the potential of nanoART for clinical use.
02:44
The Born-Haber Cycle
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2020
Lattice Energy An ionic compound is stable because of the electrostatic attraction between its positive and negative ions. The lattice energy of a compound is a measure of the strength of this attraction. The lattice energy (ΔHlattice) of an ionic compound is defined as the energy required to separate one mole of the solid into its component gaseous ions. Here, the convention is used where the ionic solid is separated into ions, meaning the lattice energies will be endothermic (positive...
06:46
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
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Cited by 58 •
2016
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
04:33
Real-Time Imaging of Macrophage Phagocytosis of IgG-Opsonized Red Blood Cells
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2025
This video demonstrates an assay to monitor independent macrophage-mediated red blood cell (RBC) phagocytosis events. Mouse immunoglobulin G (IgG)-opsonized human RBCs are incubated with mouse macrophages and observed under a confocal microscope. The RBCs are engulfed by macrophages via a phagocytic cup formation and targeted for lysosomal degradation.
16:44
Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
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Cited by 15 •
2009
We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.
FAQs
Chemokines and other signals guide circulating monocytes toward vessel walls. The cells adhere to the endothelium, the vessel lining, and then cross it to enter tissue. This sequence connects vascular signaling with the location of macrophage activity, allowing recruitment to influence where inflammation, defense, and repair processes develop.
Once monocytes enter tissue, they differentiate into macrophages with specialized capabilities. These capabilities include phagocytosis, which enables cellular or material uptake, and cytokine production, which helps coordinate immune communication. The resulting functional specialization allows local conditions to shape how the recruited cells contribute to inflammation, defense, or repair.
Recruitment determines how many circulating monocytes enter a tissue, while activation affects what those cells do after arrival. Examining both processes helps distinguish cell presence from cell behavior. In bioengineering studies, this distinction is important because a material may influence immune-cell entry, subsequent activity, or both, with consequences for tissue responses.
A bioengineering evaluation can examine whether a biomaterial, implant, or engineered tissue is associated with macrophage recruitment and activation. Researchers can then relate those immune responses to inflammation, tissue integration, and healing. Considering both recruitment and functional activity provides a broader assessment than examining the material’s physical presence alone.
Their responses provide an immune-system perspective for designing implants and engineered tissues. Researchers can use recruitment and activation patterns to identify designs that may limit chronic inflammation while supporting integration with surrounding tissue. This makes the cells relevant not only as biological responders, but also as indicators of how an engineered construct interacts with its host.
Controlling recruitment and activation offers a way to influence the balance between harmful persistent inflammation and useful repair activity. In engineered therapies, the goal is to guide macrophage behavior so that materials and tissues support controlled healing rather than prolonged immune activation. This approach connects cellular immune regulation with practical design of regenerative interventions.