Free Swelling Culture
Free Swelling Culture - Related Videos
05:53
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
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Cited by 11 •
2018
This protocol aims to describe a method to examine the Ca2+ retention capacity and Ca2+- triggered mitochondrial swelling of isolated mitochondria of SH-SY5Y cells step-by-step.
07:04
Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
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Cited by 121 •
2017
This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.
10:12
Organotypic Culture of Adult Rabbit Retina
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Cited by 8 •
2007
This article demonstrates the dissection and incubation of rabbit retina and particle-mediated gene transfer of plasmids encoding GFP or a variety of subcellular markers into retinal ganglion cells.
06:54
Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
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Cited by 12 •
2012
Traditional, two dimensional cell culture techniques often result in altered characteristics with respect to differentiation markers, cytokines and growth factors. Three-dimensional cell culture in the rotating cell culture system (RCCS) reestablishes expression of many of these factors as shown here with an extravillous trophoblast cell line.
07:06
Isolation and Primary Culture of Rat Hepatic Cells
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Cited by 137 •
2012
Primary hepatocytes provide a valuable tool to evaluate biochemical, molecular, and metabolic functions in a physiologically relevant experimental system. We describe a reliable protocol for rat in situ liver perfusion, which consistently generates viable hepatocytes up to 1.0 × 108 cells per preparation with cell viability between 88 ~ 96%.
FAQs
Water and solutes move into the construct, increasing its volume until osmotic forces are balanced by resistance from the polymer or extracellular-matrix network. This balance determines how far the material expands under immersion. The resulting equilibrium provides information about the interaction between the construct’s network structure and its surrounding culture environment.
Material composition affects the resistance of the polymer or extracellular-matrix network, which in turn influences the extent of volume change. Cells can also remodel the surrounding matrix during culture, altering the construct’s structure as swelling proceeds. Evaluating both effects helps distinguish changes caused by the biomaterial from those associated with cellular matrix formation or remodeling.
The comparison shows how external mechanical restriction changes construct development relative to conditions where natural volume changes can occur. Differences in swelling, matrix formation, or degradation can indicate how material composition and culture environment influence performance. This contrast is useful for identifying responses that may be hidden when expansion is limited by compression or confinement.
Researchers can evaluate hydration, dimensional stability, matrix formation, and degradation while the construct remains immersed in culture medium. Changes in these properties describe how the material behaves as water and solutes enter and as cells interact with the surrounding matrix. Together, the measurements provide a profile of construct development and material performance.
The approach can be applied to cells, tissues, and biomaterial constructs, making it relevant across several bioengineering research settings. Its value is greatest when natural changes in volume, matrix organization, or material integrity are important outcomes. Researchers can use the resulting observations to assess how a construct develops in a defined culture environment.
Free swelling culture provides a way to examine construct behavior without external compression or confinement, while retaining the surrounding culture medium needed for solute and water movement. In tissue engineering and biomaterials studies, the method helps connect hydration and dimensional changes with matrix formation and degradation, supporting evaluation of development and expected construct performance.