High Resolution Imaging

High resolution imaging is the capture of finely detailed visual information, allowing researchers to distinguish small structures, features, or changes that conventional imaging may miss. It achieves greater spatial resolution through optimized optics, sensitive detectors, controlled illumination, and computational image processing that preserve or reconstruct detail at small scales. In bioengineering, these methods support visualization of cells, tissues, biomaterials, and engineered devices, helping researchers measure morphology, track dynamic processes, and evaluate performance. Applications include microscopy-based disease studies, tissue engineering, diagnostic development, and the design of advanced imaging systems for quantitative biological analysis.

High Resolution Imaging - Related Videos

Research

JoVE Journal - Neuroscience

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

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Cited by 8 •

2012

This article describes techniques to perform high-resolution functional magnetic resonance imaging with 1.2 mm sampling in human midbrain and subcortical structures using a 3T scanner. Use of these techniques to resolve topographic maps of visual stimulation in the human superior colliculus (SC) is given as an example.

Super-resolution Imaging of the Bacterial Division Machinery

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Cited by 17 •

2013

We describe a super-resolution imaging method to probe the structural organization of the bacterial FtsZ-ring, an essential apparatus for cell division. This method is based on quantitative analyses of photoactivated localization microscopy (PALM) images and can be applied to other bacterial cytoskeletal proteins.

Assessing Cortical Cerebral Microinfarcts on High Resolution MR Images

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Cited by 24 •

2015

A high resolution ex vivo 7T MR imaging protocol is presented, to perform MR-guided histopathological validation of microvascular pathology in post-mortem human brain tissue. Furthermore, guidelines are provided for the assessment of cortical microinfarcts on in vivo 7T as well as 3T MR images.

Super-resolution Imaging of Neuronal Dense-core Vesicles

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Cited by 3 •

2014

We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.

Ground State Depletion Super-resolution Imaging in Mammalian Cells

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Cited by 16 •

2017

This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular proteins.

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