Human Airway Epithelium
Human Airway Epithelium - Related Videos
11:21
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
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Cited by 48 •
2010
This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.
07:40
Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium
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2025
Air-liquid interface culture is commonly used to develop pseudostratified airway epithelium by differentiating primary normal human bronchial epithelial cells that mimic the apical side of the lung airway. Here, we describe an easy protocol for determining its quality by monitoring its biophysical properties, such as ciliary function and membrane integrity.
08:44
Formation of Human Prostate Epithelium Using Tissue Recombination of Rodent Urogenital Sinus Mesenchyme and Human Stem Cells
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Cited by 10 •
2013
To unravel the earliest molecular mechanisms underlying prostate cancer initiation, novel and innovative human model systems and approaches are desperately needed. The potential of pre-prostatic urogenital sinus mesenchyme (UGSM) to induce pluripotent stem cell populations to form human prostate epithelium is a powerful experimental tool in prostate research.
08:24
A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium
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Cited by 3 •
2016
We describe a 3D culture model of the human breast epithelium that is suitable to study hormone action.
07:42
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
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Cited by 15 •
2015
The goal of this protocol is to use laser-capture micro-dissection as an effective method to isolate pure populations of cell types from heterogeneous prostate tissues for downstream RNA analysis.
FAQs
These cell types provide complementary functions rather than acting independently. Basal cells support renewal of the epithelial layer, goblet cells produce mucus that captures inhaled particles, and ciliated cells move the mucus toward the throat through coordinated beating. Bioengineered models need to represent this combination to examine barrier protection and mucociliary clearance as integrated processes.
Ciliary beating supplies the movement required to transport particle-containing mucus toward the throat. Its importance becomes clearer when considered alongside goblet-cell mucus production: mucus can trap inhaled material, while cilia provide directional transport. Reproducing both activities helps a laboratory model represent mucociliary clearance instead of examining mucus or cilia as isolated features.
Bioengineering can recreate the relevant cellular components and tissue conditions in airway organoids, engineered constructs, and other laboratory models. These formats provide distinct experimental systems for representing epithelial behavior and examining interactions with inhaled materials. The shared design goal is to reproduce important airway features sufficiently for disease, delivery, toxicity, or environmental studies.
Researchers can use these systems to investigate respiratory disease while examining how airway tissue responds to surrounding environmental conditions or inhaled materials. Because the models recreate epithelial cellular components and tissue conditions, they offer a controlled setting for studying relationships between the airway surface and its environment. This supports bioengineering research focused on disease-relevant tissue behavior.
Engineered airway systems provide laboratory platforms for evaluating inhaled drug delivery and testing potential toxicity at the respiratory epithelial interface. Their relevance comes from reproducing cellular components and tissue conditions associated with the airway surface. Consequently, researchers can study delivery or harmful effects in a model designed around human airway features rather than relying only on unrelated experimental systems.
Organoids, engineered constructs, and related laboratory models offer human airway-based systems for respiratory research, inhaled drug delivery, and toxicity testing. By recreating relevant epithelial components and tissue conditions, they can provide experimental alternatives for questions that might otherwise depend on animal models. Their value is therefore both scientific and practical, especially when studying airway-specific responses.