Journal
/
Medicine
/
Formation of Human Prostate Epithelium Using Tissue Recombination of Rodent Urogenital Sinus Mesenchyme and Human Stem Cells
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Formation of Human Prostate Epithelium Using Tissue Recombination of Rodent Urogenital Sinus Mesenchyme and Human Stem Cells

Formation of Human Prostate Epithelium Using Tissue Recombination of Rodent Urogenital Sinus Mesenchyme and Human Stem Cells

DOI:
10.3791/50327-v

•

08:44 min

•

June 22, 2013

•

, ,
1Department of Surgery, Section of Urology,University of Chicago, 2Committee on Cancer Biology,University of Chicago

Chapters

  • 00:05Title
  • 01:19Isolation of the Urogenital Sinus from Mouse or Rat Embryos
  • 02:52Separation of the Urogenital Sinus Mesenchyme
  • 05:13Transplantation of Tissue Recombinant Underneath the Renal Capsule
  • 06:57Representative Tissue Transplantation Results
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

To unravel the earliest molecular mechanisms underlying prostate cancer initiation, novel and innovative human model systems and approaches are desperately needed. The potential of pre-prostatic urogenital sinus mesenchyme (UGSM) to induce pluripotent stem cell populations to form human prostate epithelium is a powerful experimental tool in prostate research.

Tags

Rodent Urogenital Sinus MesenchymeHuman Stem CellsProstate Epithelium FormationProstate Cancer ResearchModel SystemsHuman Prostate CarcinogenesisEmbryonic Stem CellsGenetic ModificationSiRNA KnockdownRenal Capsule ImplantationInduced Pluripotent Stem Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50327?article_type=v

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model
check_url/50327?article_type=v

Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye
check_url/50327?article_type=v

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
check_url/50327?article_type=v

An Orthotopic Murine Model of Human Prostate Cancer Metastasis
check_url/50327?article_type=v

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
check_url/50327?article_type=v

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
check_url/50327?article_type=v

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
check_url/50327?article_type=v

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
check_url/50327?article_type=v

Pre-clinical Orthotopic Murine Model of Human Prostate Cancer
check_url/50327?article_type=v

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Read Article