Vesicle Encapsulation
Vesicle Encapsulation - Related Videos
05:43
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
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Cited by 13 •
2017
Giant vesicles containing highly packed micrometer-sized components are useful cell models. The water-in-oil emulsion centrifugation method is a simple, powerful tool for the preparation of giant vesicles with encapsulated materials.
10:28
The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics
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Cited by 12 •
2013
Herein we describe simple methods for the preparation of vesicles, the encapsulation of transcription and translation machinery, and the monitoring of protein production. The resulting cell-free systems can be used as a starting point from which to build increasingly complex cellular mimics.
07:48
Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles
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Cited by 20 •
2021
This article introduces a simple method for expeditious production of giant unilamellar vesicles with encapsulated cytoskeletal proteins. The method proves to be useful for bottom-up reconstitution of cytoskeletal structures in confinement and cytoskeleton-membrane interactions.
07:08
PuraMatrix Encapsulation of Cancer Cells
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Cited by 31 •
2009
This video demonstrates how to encapsulate and culture cancer cells in PuraMatrix, a commercially available self assembling peptide gel.
09:14
Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
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Cited by 10 •
2011
A method for photo-encapsulation of cells in a crosslinked PEG hydrogel is described. Hypoxic signaling within encapsulated murine insulinoma (MIN6) aggregates is tracked using a fluorescent marker system. This system allows serial examination of cells within a hydrogel scaffold and correlation of hypoxic signaling with changes in cell phenotype.
FAQs
Cargo chemistry guides its location within the vesicle. Hydrophilic molecules can be accommodated by the internal aqueous core, whereas hydrophobic molecules partition into the membrane. This partitioning principle helps bioengineers match a therapeutic, diagnostic, or biological payload with a vesicle architecture that can protect it and support the intended transport and release behavior.
Amphiphilic lipids and polymers contain chemical characteristics that support self-assembly when placed in water. Their organization produces membrane-bound compartments with an aqueous interior and, in some systems, a hydrophobic membrane region. This arrangement creates distinct locations for different cargo types and provides a material platform whose properties can be engineered for biomedical use.
Preparation conditions influence several important characteristics at once, including the size of the resulting vesicles, their stability, and the amount of cargo successfully enclosed. These measures help determine whether a formulation can maintain its structure and carry its payload effectively. Evaluating them is therefore central to optimizing vesicle-based systems for transport and release.
A basic development workflow begins by identifying the therapeutic, diagnostic, or biological cargo and considering whether it is hydrophilic or hydrophobic. The bioengineer then uses an amphiphilic lipid or polymer system in an aqueous environment to form vesicles, while adjusting preparation conditions. Vesicle size, stability, and encapsulation efficiency provide key outcomes for evaluating the formulation.
Enclosing proteins and nucleic acids within vesicles can help protect these biological cargos from degradation during transport. The membrane-bound environment also creates an opportunity to control how the payload is carried and released. In bioengineering, this protection is relevant when designing delivery systems intended to improve the performance of therapies involving fragile biological molecules.
The approach is useful for drug delivery, diagnostic cargo transport, and biological payload delivery, particularly when protection or localized release is important. Vesicles can be engineered by changing material properties and preparation conditions, allowing researchers to investigate systems suited to target tissues. These capabilities make the method relevant to developing and improving biomedical therapies.