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Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

DOI:
10.3791/55282-v

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05:43 min

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January 24, 2017

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, , , , ,
1Department of Mathematical and Physical Sciences, Faculty of Science,Japan Women’s University, 2Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience,National Institutes of Natural Sciences, 3Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science,National Institutes of Natural Sciences, 4Research Center for Complex Systems Biology,The University of Tokyo

Chapters

  • 00:05Title
  • 00:25Preparation of GVs by the W/O Emulsion Centrifugation Method
  • 03:06Microscopy Observations of GVs
  • 03:56Results: Water-in-oil Emulsion Method for Preparation of GVs with Encapsulated Materials
  • 05:12Conclusion

Summary

Automatic Translation

Automatic Translation

Giant vesicles containing highly packed micrometer-sized components are useful cell models. The water-in-oil emulsion centrifugation method is a simple, powerful tool for the preparation of giant vesicles with encapsulated materials.

Tags

Keywords: Giant VesiclesMicrospheresWater-in-oil EmulsionCentrifugationArtificial CellDOPCTexas Red DHPELipid FilmLiquid ParaffinSucroseTris BufferFluorescent MicrospheresHomogenizerCentrifugation

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