Cardiomyocyte Transduction
Cardiomyocyte Transduction - Related Videos
08:42
Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes
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Cited by 35 •
2016
This protocol describes a step-by-step method for the reproducible isolation and long-term culture of adult mouse cardiomyocytes with high yield, purity, and viability.
13:36
Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy
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Cited by 12 •
2014
This protocol describes a method of live cell imaging using primary rat neonatal cardiomyocytes following lentiviral and adenoviral transduction using confocal spinning disk microscopy. This enables detailed observations of cellular processes in living cardiomyocytes.
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
08:03
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
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Cited by 25 •
2016
Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.
07:05
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
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Cited by 49 •
2015
The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.
FAQs
Viral and nonviral vectors provide alternative ways to move genetic cargo across the cardiomyocyte membrane. The source material identifies both as delivery options but does not specify that one is universally superior. Their selection can therefore be matched to the experimental objective, cargo type, desired selectivity, and need to study cardiac gene function or therapeutic strategies.
Membrane entry alone does not establish a biological effect. After delivery, intracellular trafficking moves DNA, RNA, or genome-editing components toward the site where they can act and alter gene expression. This step helps determine whether introduced material becomes functionally available, making trafficking an important part of interpreting successful or limited transduction outcomes.
The described approaches can deliver DNA, RNA, or genome-editing components into cardiomyocytes. These cargo classes support different experimental goals, including altering gene expression, examining gene function, or testing genome-directed strategies. Connecting the cargo with its intended intracellular site of action is essential for producing a measurable biological effect in cardiac studies.
Effectiveness depends on how efficiently and selectively the delivery system introduces cargo, followed by whether intracellular trafficking brings it to the appropriate site of action. These features influence the consistency of gene-expression changes and the clarity of experimental results. Improving delivery can therefore strengthen reliability when studying cardiac biology or evaluating gene-based strategies.
A study begins by selecting genetic cargo and a viral or nonviral delivery approach suited to the question. The material is introduced across the cardiomyocyte membrane, then intracellular trafficking carries it toward its site of action. Researchers can subsequently examine the resulting biological effect, such as altered gene expression or changes relevant to cardiac disease.
Researchers apply this technique to investigate cardiac gene function, model inherited and acquired heart disease, test therapeutic strategies, and support cardiac-regeneration studies. These applications connect controlled genetic manipulation with questions about heart-muscle biology. In each case, efficient and selective delivery helps make the resulting observations more interpretable and supports evaluation of gene-based treatment concepts.