Plant Pathogen Infection

Plant pathogen infection is the invasion and colonization of plant tissues by disease-causing organisms or agents, a process that disrupts normal growth and can reduce crop productivity. It begins when a pathogen reaches a susceptible host, overcomes physical and chemical defenses, and establishes itself through tissue entry, cellular exploitation, or transmission between cells. Plants respond with defense signaling, antimicrobial compounds, and localized cell death, while pathogens may alter host physiology to obtain nutrients and reproduce. Studying plant pathogen infection helps biologists explain disease development, host resistance, and pathogen spread, supporting crop protection, breeding resistant varieties, and developing diagnostic and management strategies.

Plant Pathogen Infection - Related Videos

Research

JoVE EoE - Bacterial Growth and Techniques

Extraction of Genomic DNA from Plant Leaf Tissue Infected with Pathogenic Bacteria

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2025

Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This protocol extracts genomic DNA from Yersinia ruckeri by suspending isolated colonies in ultrapure water and applying heat to lyse the cells. Centrifugation separates the DNA-containing supernatant from cell debris, yielding template DNA suitable for PCR-based genotyping and epidemiological analysis.

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

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Cited by 9 •

2018

Here, we present a protocol to test plant virulence with the plant pathogen Magnaporthe grisea. This report will contribute to the large-scale screening of the pathotypes of fungi isolates and serve as an excellent starting point for understanding the resistant mechanisms of plants during molecular breeding.

Research

JoVE Journal - Immunology and Infection
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Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

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Cited by 23 •

2009

A cell death-based assay for PTI in Nicotiana benthamiana plants is described.

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

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Cited by 234 •

2012

Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella typhimurium and Mycobacterium marinum. Micro-injection of bacteria and multi-color fluorescence imaging are essential techniques involved in the application of zebrafish embryo infection models.

Single-Cell Analysis of Gene Expression from Plant-Extracted Pathogens

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2026

Source: Rufián, J. S., et al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022).This video demonstrates a method for analyzing gene expression in plant-extracted pathogenic bacteria at the single-cell level using a fluorescently tagged transcription factor. Confocal imaging reveals heterogeneity in virulence gene expression, highlighting functional diversity within a clonal bacterial population.

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