Calvarial Window
Calvarial Window - Related Videos
03:15
Calvarial Window Generation in Mouse Model: A Surgical Procedure to Create Window in the Top Part of the Mouse Skull
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2023
In this video, we describe the surgical procedure to create a calvarial window for accessing the brain of a mouse model.
11:31
Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
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Cited by 21 •
2012
This protocol describes the isolation of adipose-derived stromal cells from lipoaspirate and the creation of a 4 mm critical-sized calvarial defect to evaluate skeletal regeneration.
09:46
Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening
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Cited by 9 •
2018
This manuscript describes a murine calvarial osteolysis model by exposure to CoCrMo particles, which constitutes an ideal animal model for assessing the interactions between wear particles and various cells in aseptic loosening.
15:01
Windowing Chicken Eggs for Developmental Studies
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Cited by 37 •
2007
The ease of accessibility of the embryo has allowed for experiments to map cell fates using several approaches, including chick quail chimeras and focal dye labeling. In this article we demonstrate one egg preparation method that has been optimized for long survival times.
09:12
Dendra2 Photoswitching through the Mammary Imaging Window
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Cited by 63 •
2009
Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.
FAQs
The main experimental advantage is repeated observation of the same tissue in a living animal over time. This longitudinal design lets investigators follow changes in tumor growth, angiogenesis, invasion, blood flow, and immune-cell interactions within one imaging field. Because each animal can serve as its own temporal reference, the approach reduces animal-to-animal variation when tumor behavior or treatment effects are compared.
A sterile coverslip serves two linked purposes: it protects the exposed dura after the calvarial bone has been removed or thinned, and it helps secure the window. Securing the window stabilizes the imaging field, which is essential when investigators return to observe the same tissue repeatedly. This supports consistent tracking of tumor-associated changes over time.
Intravital microscopy can follow several dimensions of tumor behavior within the same preparation. Researchers may examine tumor growth and invasion alongside angiogenesis, blood flow, and interactions with immune cells. Considering these features together allows observation of structural tumor changes in relation to vascular and immune activity, rather than limiting interpretation to a single measurement collected at one endpoint.
A stable field allows observations from successive imaging sessions to be compared more consistently. Investigators can examine whether therapy changes tumor growth, angiogenesis, invasion, blood flow, or immune-cell interactions in the same monitored region. This longitudinal comparison helps distinguish treatment-associated changes from differences that would otherwise arise between separate animals.
The preparation involves surgically removing or thinning a small area of calvarial bone, protecting the exposed dura with a sterile coverslip, and securing the window to stabilize the imaging field. These steps create a protected access site for repeated intravital microscopy. The resulting preparation supports observation of tumor-associated processes in living animals over time.
Researchers would use this approach when they need to monitor tumor behavior and its surrounding environment repeatedly in living animals. It is suited to studies of tumor growth, angiogenesis, invasion, blood flow, and immune-cell interactions, as well as evaluations of how therapies alter these features. Repeated measurements can reduce animal-to-animal variation during treatment comparisons.