Journal
/
Biology
/
Dendra2 Photoswitching through the Mammary Imaging Window
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Dendra2 Photoswitching through the Mammary Imaging Window

Dendra2 Photoswitching through the Mammary Imaging Window

DOI:
10.3791/1278-v

•

09:12 min

•

June 05, 2009

•

, , , ,
1Department of Anatomy and Structural Biology,Albert Einstein College of Medicine – Yeshiva University, 2Gruss Lipper Biophotonics Center,Albert Einstein College of Medicine – Yeshiva University, 3Hubrecht Institute-KNAW and University Medical Center Utrecht

Chapters

  • 00:05Title
  • 00:25Introduction
  • 01:03Manual Fabrication of the Mammary Imaging Window Window (MIW)
  • 02:58Insertion of MW
  • 05:28Using the Imaging Box
  • 06:39Photoswitching and Imaging of Dendra2-labeled Cells
  • 08:09Representative Results: Photoswitching
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.

Tags

Intravital MicroscopyBreast TumorsMiceRatsSingle-cell ResolutionMetastatic BehaviorMigrationInvasionIntravasationAngiogenesisImmune Cells ResponseMammary CarcinomasMammary Imaging Window (MIW)Imaging ParametersDendra2 PhotoswitchingIn Vivo ImagingProtocolManufacturing MIWTumor Cells ImagingCustom Built Imaging BoxMetastatic Behavior

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1278?article_type=v

A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window
check_url/1278?article_type=v

Proper Care and Cleaning of the Microscope
check_url/1278?article_type=v

Major Components of the Light Microscope
check_url/1278?article_type=v

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
check_url/1278?article_type=v

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
check_url/1278?article_type=v

Mammary Epithelial Transplant Procedure
check_url/1278?article_type=v

Functional Imaging of Brown Fat in Mice with 18F-FDG micro-PET/CT
check_url/1278?article_type=v

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland
check_url/1278?article_type=v

Imaging Through the Pupal Case of Drosophila melanogaster
check_url/1278?article_type=v

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Read Article