Ultra-low Adhesion Plates
Ultra-low Adhesion Plates - Related Videos
02:47
In Vitro Parallel-Plate Flow Model to Study Bacterial Adhesion on a Tissue Biopsy
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2026
Source: Ditkowski, B., et al. An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues. J. Vis. Exp. (2019).This video demonstrates the method of using a parallel-plate flow chamber to perfuse fluorescently labeled bacteria over vascular graft tissues, enabling controlled analysis of bacterial adhesion and early pathogenesis under shear flow conditions.
03:10
Micro-Parallel Plate Flow Chamber Model for Studying Bacterial Adhesion to Human Endothelial Cells
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2026
Source: Claes, J., et al, In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions. J. Vis. Exp. (2015).This video demonstrates a method for studying host-pathogen interactions using a micro-parallel plate flow chamber seeded with human primary endothelial cells. Following endothelial cell activation by an ionophore and perfusion with fluorescently labeled bacteria, the cells release von Willebrand factor (VWF), which binds bacterial adhesins under shear...
01:14
Adhesion
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2019
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules. Capillary action is a result of water’s adhesive tendencies. When a narrow glass...
07:40
Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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Cited by 3 •
2016
This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.
04:36
Assay for Adhesion and Agar Invasion in S. cerevisiae
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Cited by 17 •
2006
We describe a qualitative assay for yeast adhesion and agar invasion as a measure of invasive and pseudohyphal differentiation. This simple assay can be used to assess the invasive phenotype of various mutants as well as the effects environmental cues and signaling pathways on yeast differentiation.
FAQs
Their non-adhesive coatings reduce interactions between cell adhesion molecules and the vessel surface. With less opportunity to attach and spread across the plate, cells remain in suspension and can interact with one another, encouraging aggregation into spheroids. This surface-driven change shifts the culture away from conventional two-dimensional growth and toward a three-dimensional organization.
Conventional monolayer cultures encourage cells to attach and spread across a surface, whereas Ultra-low Adhesion Plates support suspension and three-dimensional assembly. This distinction matters because tumor spheroids can better represent aspects of three-dimensional tumor organization. As a result, the plates complement monolayer assays when researchers want to examine cancer-cell behavior in a different culture arrangement.
Spheroids generated in Ultra-low Adhesion Plates provide models for investigating tumor-cell behavior, growth, and invasion. They also allow researchers to examine how these three-dimensional assemblies respond to anticancer treatments. Studying several outcomes in the same type of model helps connect spheroid organization with cancer-relevant responses that may not be captured fully by monolayer cultures.
Reducing surface attachment changes the main physical interaction available to cultured cells. Instead of primarily spreading across the vessel, cells are encouraged to remain together and form aggregates. In cancer research, that organization supports tumor-spheroid models, allowing investigators to study cell behavior within a three-dimensional assembly rather than only across a flat culture surface.
Researchers place cells into the engineered vessels, where reduced surface attachment supports suspension and cell aggregation. The resulting spheroids can then be maintained for investigations of tumor-cell behavior, growth, invasion, or treatment response. The workflow therefore connects vessel selection with model formation and subsequent observation or treatment in a three-dimensional cancer-culture system.
This approach is useful when the research question concerns three-dimensional tumor organization or responses within tumor spheroids. It can support studies of growth, invasion, cell behavior, and anticancer treatment response while complementing conventional monolayer assays. Using both formats gives preclinical research access to different culture contexts rather than requiring one model to represent every tumor feature.