Mouse Hepatocyte Transfection
Mouse Hepatocyte Transfection - Related Videos
03:48
Hydrodynamic Gene Delivery: A Technique for In Vivo Transfection of Exogenous DNA in Murine Hepatocytes via Tail Vein Injection
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2025
This video describes the technique of genetic in vivo transfection of murine hepatocytes by hydrodynamic tail vein injection. This transfection system generates a long-term expression of the transgene in the hepatocytes.
04:21
Protein Transfection of Mouse Lung
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Cited by 1 •
2013
Transgenic mice or viral vectors have been used to increase protein expression within the lung. However, these techniques are time-consuming, technically challenging and have off-target effects that can confound results. Our protein transfection protocol uses a lipid based transfection reagent and an ultrafine microsprayer to uniformly deliver active protein to lung cells.
12:11
Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes
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Cited by 39 •
2015
De novo lipogenesis and β-fatty acid oxidation constitute key metabolic pathways in hepatocyte, pathways that are perturbed in several metabolic disorders, including fatty liver disease. Here we demonstrate isolation of mouse primary hepatocytes and describe quantification of β-fatty acid oxidation and lipogenesis.
05:26
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
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Cited by 5 •
2011
We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.
09:30
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
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Cited by 49 •
2011
A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development.
FAQs
Delivery reagents and physical methods temporarily overcome the hepatocyte plasma membrane, creating access to the cell interior. Once inside, DNA, RNA, or gene-editing components may act in the cytoplasm, while DNA and appropriate editing components can reach the nucleus. This compartmental distinction helps determine whether an experiment primarily examines expression, silencing, or genetic modification.
The nucleic acid type determines the biological question the experiment can address. DNA can support gene expression and regulatory-sequence studies when it reaches the nucleus, whereas RNA is suited to examining gene silencing or cytoplasmic effects. Gene-editing components are selected when researchers want to investigate modification-related outcomes or variant-specific effects in hepatocytes.
Mouse hepatocytes provide a physiologically relevant cellular setting for connecting genetic changes with hepatocyte function. This context supports analysis of regulatory sequences and metabolic pathways while preserving a direct relationship between molecular events and liver-cell behavior. The system therefore helps researchers move beyond isolated sequence measurements toward functional interpretation of genetic effects.
Planning begins with matching the introduced nucleic acid to the intended outcome, such as expression, silencing, or genetic modification. Researchers must also select a delivery reagent or physical method capable of crossing the plasma membrane and consider whether activity requires cytoplasmic access or nuclear entry. These choices shape how clearly molecular changes can be linked to hepatocyte function.
A general workflow starts by selecting DNA, RNA, or gene-editing components for the genetic question, followed by delivery into mouse hepatocytes with a reagent or physical method. The resulting molecular change is then examined in relation to gene function, regulatory activity, metabolism, silencing, or variant-specific behavior. This sequence keeps the intervention aligned with the desired biological interpretation.
These studies are useful when researchers need a controlled platform to examine how introduced genetic material or editing components affect liver cells. Mouse hepatocytes can connect the intended molecular intervention with changes in gene activity and cellular function, making the approach relevant to preliminary evaluation of genetic therapies. They also support comparison of variant-specific effects before broader interpretation.