Microsphere Phagocytosis
Microsphere Phagocytosis - Related Videos
04:03
An In Vitro Assay to Measure Antibody-Dependent Cellular Phagocytosis by Phagocytes
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2025
This video demonstrates an assay to measure the antibody-dependent cellular phagocytosis (ADCP) by breast milk phagocytes. Fluorescently labeled microspheres conjugated to an antigen-antibody complex, are combined with a cell suspension isolated from breast milk to induce antibody-dependent cellular phagocytosis by the phagocytic leukocytes. Upon labeling the cells with antibodies against common leukocyte antigen (CD45), flow cytometry is used to measure the fluorescence intensity of...
08:06
Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications
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Cited by 3 •
2017
Protocols for the synthesis of microspheres from polymers, the manipulation of microspheres, and micro-photoluminescence measurements are presented.
03:02
Microsphere Polymerase Chain Reaction to Amplify Single-Stranded DNA
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2025
This video describes the technique of amplifying single-stranded DNA using oligonucleotide-coated polymer microspheres in a single-tube reaction. This process yields pure single-stranded DNA, which can be further used for DNA sequencing and microarray analysis.
03:54
Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres
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2025
Source:Rhea Kang1, Gabby Mora1, Sherry Dunbar11Luminex, A DiaSorin Company, Austin.This video demonstrates multiplexed quantitative bacterial detection employing carboxylated magnetic polystyrene microspheres dyed into distinct sets. The procedure encompasses the formation of bead-bacteria complexes, magnetic separation, and flow-based analysis, enabling precise and simultaneous quantification of multiple bacteria in the same reaction well.
09:29
Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
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Cited by 10 •
2014
This protocol combines electrospinning and microspheres to develop tissue engineered scaffolds to direct neurons. Nerve growth factor was encapsulated within PLGA microspheres and electrospun into Hyaluronic Acid (HA) fibrous scaffolds. The protein bioactivity was tested by seeding the scaffolds with primary chick Dorsal Root Ganglia and culturing for 4-6 days.
FAQs
Surface-receptor engagement determines whether microspheres are recognized efficiently, while opsonization can enhance that recognition. Opsonization means coating a particle with factors that make it more readily detected by phagocyte receptors. This makes receptor availability and particle coating important experimental variables when investigators compare macrophage or neutrophil uptake or examine altered innate immune responsiveness.
Following receptor recognition, actin rearrangement helps the cell surround the microsphere and close it within an intracellular phagosome. That compartment is not the endpoint: it can proceed toward phagolysosomal processing, where the internalized material is handled after uptake. Tracking these stages connects measured particle entry with the cellular machinery underlying phagocytic activity.
Phagocytic output can vary with receptor function, immune-cell activation, drug exposure, and inflammatory conditions. Microsphere assays allow investigators to compare how these factors alter uptake by macrophages or neutrophils. The resulting differences can indicate changes in innate immune responsiveness, although the assay uses a synthetic particle rather than a microorganism.
Because the particles are synthetic yet provide bead-like targets that phagocytes can recognize and internalize, the model offers a measurable way to investigate pathogen-like particle clearance. It can link receptor activity and cell activation to quantifiable uptake, supporting comparisons of cellular responses in immunology and infection research.
Begin by considering the interaction between microspheres and the selected phagocytes, including receptor recognition and any opsonization used to aid binding. The cells then reorganize actin, enclose particles in phagosomes, and process them in phagolysosomal compartments. Investigators finally quantify uptake with microscopy, flow cytometry, or fluorescence measurements to compare phagocytic activity.
Microscopy, flow cytometry, and fluorescence measurements are the principal readouts identified for this model. These approaches allow investigators to convert particle internalization into a measurable assessment of phagocytic activity and compare receptor function, cell activation, drug effects, or inflammatory conditions. The readout connects cellular processing with an experimentally observable outcome.
Applications include assessing phagocyte receptor function, innate immune-cell activation, pathogen-like particle clearance, and the influence of drugs or inflammatory conditions. Macrophages and neutrophils provide relevant cellular systems because both can recognize microspheres through surface receptors. Results can help characterize how altered receptor-dependent recognition or immune state changes particle uptake and clearance responses.