Aspartate Aminotransferase
Aspartate Aminotransferase - Related Videos
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Cited by 3 •
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The αvβ3 integrin is a type of adhesion protein that is highly expressed on activated endothelial cells undergoing angiogenesis. Thus, evaluating the integrity of the integrin is of great interest in oncology. Here, we introduce a method to prepare 68Ga-labeled radiopeptides and a method to assess its biological effectiveness.
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07:11
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2025
Here, we describe the use of a genetically directed alkaline phosphatase (AP) labeling method to investigate the anatomical features and phenotypes of mouse retinas under NMDA-induced excitotoxicity. In conjunction with several retinal ganglion cell (RGC) subtype-specific reporter lines, we uncover novel phenotypes in dying RGCs throughout the degenerative process.
07:20
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
0 Views •
Cited by 1 •
2018
We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.
06:00
Laparoscopic Anatomic S7+S8d Resection Preserving Inferior Right Hepatic Vein and S6 with Right Hepatic Vein Transection
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2025
We present a laparoscopic technique for anatomic S7+S8d resection with right hepatic vein transection, preserving the inferior right hepatic vein and segment S6. Relying on meticulous anatomical landmarks without intraoperative ultrasound, this approach offers a precise and parenchyma-sparing option for dorsally located right-liver tumors, particularly in settings with limited advanced navigation resources.
FAQs
Pyridoxal phosphate supports AST activity during transamination. In this reaction, the enzyme transfers an amino group between aspartate and alpha-ketoglutarate, producing oxaloacetate and glutamate. This biochemical role connects AST measurement with cellular amino acid metabolism, while changes in circulating activity provide information about injury affecting tissues that contain the enzyme.
AST is distributed across several tissues, including the liver, heart, skeletal muscle, kidneys, and red blood cells. Consequently, an increased blood level does not identify the liver as the only possible source of cellular injury. Its broad tissue distribution makes interpretation dependent on other laboratory findings and the clinical context rather than the AST value alone.
Comparing AST with alanine aminotransferase helps place an AST result in a broader pattern of laboratory findings. Because AST occurs in multiple tissues, this comparison can contribute to assessing whether liver injury is likely and whether another source of cellular damage should be considered. Interpretation with additional laboratory results strengthens clinical assessment compared with using AST in isolation.
Clinicians use AST measurement as part of an assessment for tissue injury, particularly when evaluating possible liver involvement. Results can also support disease monitoring over time. The test does not independently establish a specific cause, so its clinical value comes from combining the result with alanine aminotransferase and other laboratory findings.
An elevated AST level can indicate injury in tissues where the enzyme is abundant, including hepatic, cardiac, skeletal muscle, renal, or red blood cell compartments. The result therefore signals possible cellular damage but does not by itself identify the affected tissue. Additional laboratory findings are needed to distinguish among these potential sources during medical evaluation.
Red blood cells contain AST, so cellular injury involving this compartment can contribute to an increased blood level. This is clinically relevant because the finding may not represent liver injury alone. Considering red blood cells alongside the liver, muscle, heart, and kidney sources helps clinicians avoid assigning a single tissue origin without supporting laboratory evidence.