High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

The overall goal of the following experiment is to detect neuronal anti D two R or N-M-D-A-R antibodies in patient samples using a high-throughput flow cytometry cell-based assay. This is achieved by sub cling, the full CDNA sequence of human D two R or N-M-D-A-R within a plasmid. As a second step, a human cell line is transfected, which leads to the cell surface expression of neuronal antigens.

Next transfected cells are incubated with patient serum and an appropriate secondary antibody before flow cytometry acquisition. In order to detect antibody binding to cell surface antigens, results are obtained based on flow cytometry analysis. This Our high three port flow cytometry cell-based assay is fast, quantitative and efficient for large cohorts of patient samples.