High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

Mazen Amatoury; Vera Merheb; Jessica Langer; Xin Maggie Wang; Russell Clive Dale; Fabienne Brilot

doi:10.3791/50935

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High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

JoVE Journal
Medicine

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JoVE Journal Medicine

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

16,150 Views

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10:19 min

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November 23, 2013

DOI:

10.3791/50935-v

DOI:

10.3791/50935-v

•

10:19 min

November 23, 2013

•

6 Views

Mazen Amatoury¹, Vera Merheb¹, Jessica Langer¹, Xin Maggie Wang², Russell Clive Dale¹, Fabienne Brilot¹

¹Institute for Neuroscience and Muscle Research, The Kids Research Institute at the Children’s Hospital at Westmead,The University of Sydney, ²Flow Cytometry Centre,Westmead Millennium Institute for Medical Research

Summary

Over the recent years, live cell-based assays have been used successfully to detect antibodies against surface and conformational antigens. Here, we describe a method using high-throughput flow cytometry enabling the analysis of large cohorts of patients. Detection of novel antibodies will improve diagnosis and treatment of immune-mediated disorders.