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A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
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Neuroscience
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JoVE Journal Neuroscience
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood

DOI:
10.3791/56676-v

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07:20 min

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January 09, 2018

•

,
1Department of Psychiatry,Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences,Chang Gung University

Chapters

  • 00:05Title
  • 00:37Transfection of HEK293 Cells with NR1-GFP Expression Plasmid
  • 02:38Cell-based Immunofluorescence Assay
  • 04:41Results: Anti-NMDA Receptor Autoantibodies Detected in the Blood of Patients with Autoimmune Encephalitis
  • 06:12Conclusion

Summary

Automatic Translation

Automatic Translation

We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.

Tags

Keywords: Cell-based Immunofluorescence AssayNMDA ReceptorAutoantibodyAutoimmune EncephalitisHEK293 CellsNR1-GFP PlasmidTransfectionFluorescence MicroscopyParaformaldehydePBSTSkim Milk

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