Porcine Tissue Sampling

Porcine tissue sampling is the controlled collection of tissue specimens from pigs for diagnostic, pathological, physiological, or biomedical research, making it essential for linking tissue changes to disease and treatment outcomes. The process involves selecting representative sites, collecting samples with appropriate instruments under aseptic conditions, and preserving them by fixation or rapid handling for downstream analysis. In medicine, samples may support histopathology, microbiology, molecular testing, and evaluation of drug safety or therapeutic response. Consistent sampling methods help maintain tissue integrity, reduce contamination, and produce reliable data for veterinary studies, translational research, and the development of human medical interventions.

Porcine Tissue Sampling - Related Videos

Research

JoVE Journal - Medicine

Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models

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Cited by 20 •

2018

The practical application and performance of methods for generation of representative tissue samples of porcine animal models for a broad spectrum of downstream analyses in biobank projects are demonstrated, including volumetry, systematic random sampling, and differential processing of tissue samples for qualitative and quantitative morphologic and molecular analyses types.

Pig Bronchiolar Tissue Extraction: A Procedure to Dissect Bronchiolar Tissue Sections From Porcine Lungs

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2025

In this video, we describe a surgical procedure to extract bronchiolar tissues from harvested pig lung. The ex vivo bronchiolar tissue could be used to develop infection models for studying chronic lung infections.

Research

JoVE Journal - Developmental Biology
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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

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Cited by 47 •

2016

This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

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Cited by 1 •

2018

Here we demonstrate a protocol to standardize sampling procedures of an established porcine model of acute myocardial infarction in order to increase its translational value in the understanding of the pathophysiology of myocardial ischemia/reperfusion injury and to test novel drug candidates.

Generation of Porcine Ventricular Slices: A Vibratome-based Technique to Prepare Ultra-thin Slices of Pig Heart Tissue

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2025

This video demonstrates a vibratome-based procedure to generate viable, ultra-thin sections of pig heart. The prepared tissue slices can be cultured and used as model systems for pharmacological testing.

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