Serial Transverse Sectioning
Serial Transverse Sectioning - Related Videos
09:46
A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
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Cited by 15 •
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This study presents reliable and easy procedures for obtaining serial ultrathin sections of a microorganism without expensive equipment in transmission electron microscopy.
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Transverse Aortic Constriction in Mice
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Transverse aortic constriction (TAC) in the mouse is a commonly used experimental model to study mechanisms underlying cardiac hypertrophy and heart failure development. Here, we describe procedures to constrict the aorta to create a reproducible degree of cardiac hypertrophy in mice.
10:53
Serial Dilutions and Plating: Microbial Enumeration
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2023
Source: Jonathan F. Blaize1, Elizabeth Suter1, and Christopher P. Corbo1 1 Department of Biological Sciences, Wagner College, 1 Campus Road, Staten Island NY, 10301 Quantitative assessment of prokaryotes can be onerous given their abundance, propensity for exponential proliferation, species diversity within a population, and specific physiological needs. Compounding this challenge, is the four-phase nature in which bacteria replicate (lag, log, stationary and death). The ability to accurately...
11:19
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
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Cited by 12 •
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This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.
07:40
Minimally Invasive Transverse Aortic Constriction in Mice
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Cited by 42 •
2017
Minimally invasive transverse aortic constriction (MTAC) conserves the essentials of regular transverse aortic constriction (TAC) while eliminating the use of a ventilator with tracheal intubation. It proves to be a highly desirable method for high-throughput studies on left ventricular overload, particularly in translational studies.
FAQs
Successive depths preserve the order in which anatomical features appear, change, and disappear through a specimen. This depth relationship allows investigators to follow lesions, vessels, and cellular patterns rather than interpreting each microscopic field in isolation. The resulting sequence is especially valuable when a structure’s shape or spatial connection cannot be understood from a single section.
Cutting across the short axis provides repeated views through the specimen’s depth, making it possible to compare the same region across neighboring levels. As structures change between sections, their position and spatial relationships become more apparent. In medical analysis, this orientation can clarify how vessels, lesions, and other features are distributed within complex tissues.
Alignment preserves correspondence between consecutive sections so that features can be tracked from one level to the next. When sections are arranged in sequence, their two-dimensional information can support a three-dimensional reconstruction. This helps distinguish genuine changes in tissue organization from differences that might otherwise be overlooked when sections are examined separately.
The specimen is first fixed and embedded, then cut at successive depths. Each resulting section is collected and mounted for examination, and staining may be applied to improve the visibility of tissue features. Maintaining the sequence during collection and preparation is important because the analytical value depends on relating each section to its position through the specimen.
Mounting keeps each thin section available for microscopic analysis, while staining can make cellular patterns and other tissue features easier to examine. Because the sections remain associated with successive depths, visual information from individual slides can be compared across the series. This combination supports more consistent interpretation of anatomical organization and pathological change.
The method is useful for studying pathology, developmental anatomy, and spatial relationships within complex tissues. It can show how lesions, vessels, and cellular patterns vary through a specimen rather than only at one level. When consecutive sections are aligned, the series can also provide a basis for three-dimensional reconstruction and more detailed anatomical interpretation.