Vasal Vessel Preservation

Vasal vessel preservation is a microsurgical principle that protects the blood vessels accompanying the vas deferens during procedures involving vasectomy, vasectomy reversal, or other male reproductive reconstruction. Surgeons carefully identify the deferential vessels and limit dissection, traction, and cautery so collateral blood flow to the vasal tissue and surrounding structures remains intact. Maintaining this vascular supply may reduce ischemic injury, support healing, and help preserve tissue quality for microsurgical reconstruction. The technique is therefore relevant to operative planning, fertility restoration, and research on how vascular handling influences outcomes in male reproductive surgery.

Vasal Vessel Preservation - Related Videos

Research

JoVE Journal - Medicine

An Experimental Human DIEP Flap Model to Investigate Preservation Strategies for Vascularized Composite Allografts and Free Flaps

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2025

This protocol describes the surgical technique for harvesting a human perforator flap model based on the deep inferior epigastric artery pedicle, intended for experimental research.

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

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Cited by 1 •

2017

This work demonstrates a novel approach to assess the proliferation, differentiation, and vessel-forming potential of vascular endothelial stem cells (VESCs) through mammary fat pad transplantation followed by whole-mount tissue preparation for microscopic observation. A lineage tracing strategy to investigate the behavior of VESCs in vivo is also presented.

Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

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Cited by 11 •

2015

We herein describe the method of fibered confocal fluorescent microscopy (FCFM) based imaging, which provides an innovative mode to understand physiological phenomena at the cellular and sub-cellular levels in animal subjects.

Calcified Artery Preparation and Processing with Preserved Morphology and RNA for Digital Spatial Profiling

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2026

This protocol includes a step-by-step workflow for calcified vascular specimens: tissue handling, decalcification, RNA validation, calcification level detection, and region-of-interest selection strategies on the Nanostring GeoMx Digital Spatial Profiler (DSP). The goal is to present a comprehensive method for preserving vascular tissue morphology and RNA for reliable spatial transcriptomic analysis.

Purification of Mouse Brain Vessels

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Cited by 90 •

2015

We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.

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