Fluorescent Dye Staining
Fluorescent Dye Staining - Related Videos
03:38
hFTAA Staining of Amyloid Deposits: A Technique to Visualize Amyloid Fibrils Using Oligothiophene-Based Dye for Fluorescence Imaging in Tissue Sections
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2025
This video describes a technique to stain amyloids in tissue sections using a luminescent-conjugated oligothiophene dye - heptamer-formyl thiophene acetic acid, or hFTAA - for fluorescence imaging. Amyloids are β-sheet-rich abnormal protein aggregates. Their detection in tissue samples is helpful in the diagnosis and treatment of amyloid-associated diseases or proteinopathies.
22:49
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
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Cited by 45 •
2012
Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
10:53
Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes
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Cited by 5 •
2009
We described structural features of the Glia-neuromuscular synapses in a novel Inside-out tissue preparation of live fly larvae using fluorescent dyes with confocal microscopy. We labeled live neuron terminals with fluorescent primary antibodies to HRP, and also visualized the perisynaptic space with fluorescent Dextrans.
04:47
Retrograde Labeling of Drosophila Motor Neurons Using Fluorescent Lipophilic Dyes
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2025
Source: Inal, M. A. et. al., Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes. J. Vis. Exp. (2020)This video demonstrates the method of retrograde labeling of Drosophila motor neurons using lipophilic dyes. The dye enters the axon, travels retrogradely, and labels the neurons green, allowing detailed visualization of their morphology and projections under a confocal microscope.
03:10
Optical Recording of Neuronal Activity in Brain Slices Stained with a Voltage-Sensitive Dye
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2025
This video demonstrates a procedure for observing changes in neuronal activity in a brain slice stained with a voltage-sensitive dye. The dye reflects changes in the neuron's membrane potential by altering its fluorescence intensity. Through the application of electrical stimuli and advanced imaging techniques, this process enables visual tracking of real-time changes in neuronal membrane potential based on VSD fluorescence.
FAQs
Fluorophores first absorb light at one wavelength and then emit light at a longer wavelength. This shift enables fluorescence microscopy to distinguish emitted signal from the illumination used to excite the molecules. The resulting signal makes labeled cellular or molecular features visible, allowing researchers to examine where selected structures occur within neural samples.
Chemical properties can direct a dye toward particular cellular features, while attached probes provide more specific localization to targets such as neuronal membranes, proteins, or nucleic acids. This targeting determines which structures produce fluorescence. Selecting labels according to the biological question therefore helps connect the observed signal with a defined neuronal component or molecular distribution.
Using distinct dyes allows researchers to visualize different structures or target types within the same broader sample. In neuroscience, this can support comparisons among neuronal morphology, cell organization, and molecular distributions. When the signals are paired with imaging and quantitative analysis, researchers can assess spatial relationships and compare structural or molecular changes across samples.
The method can be applied to both fixed and cultured samples, supporting different kinds of neural investigation. Fixed material permits examination of preserved tissue organization, whereas cultured samples provide a setting for observing cellular features in a maintained experimental system. In either context, fluorescence can reveal morphology, organization, or molecular localization relevant to the study.
A typical conceptual workflow selects a dye or attached probe for the target, applies it to a fixed or cultured neural sample, examines the resulting signal with fluorescence microscopy, and performs quantitative analysis. The choice of label establishes what can be visualized, while imaging and measurement convert fluorescence patterns into comparisons of cellular or molecular features.
Fluorescent dye staining can help researchers examine neuron morphology, cell organization, and the distribution of proteins or nucleic acids. These observations support investigations of neural development, connectivity, and mechanisms underlying neurological disease. Comparing labeled structures or signals across samples can reveal cellular changes and provide measurable evidence for differences in neural organization.