Plla Scaffolds
Plla Scaffolds - Related Videos
04:33
Decellularized Porcine Esophageal Scaffold Preparation: A Technique to Generate Acellular Scaffold from a Pig Esophagus
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2023
In this video, we demonstrate the preparation of a decellularized pig esophagus scaffold, with its biochemical architecture and composition preserved, for tissue engineering applications.
09:32
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
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Cited by 5 •
2015
Here, we present a protocol to fabricate electrospun nanofiber scaffolds with gradated organization of fibers and explore their applications in regulating cell morphology/orientation. Gradients with regard to physical and chemical properties of the nanofiber scaffolds offer a wide variety of applications in the biomedical field.
08:35
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
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Cited by 21 •
2011
Elastomeric PGS scaffolds with vascular smooth muscle cells cultured in a pulsatile flow bioreactor may lead to promising small-diameter arterial constructs with native ECM production in a relatively short culture period.
14:49
Self-reporting Scaffolds for 3-Dimensional Cell Culture
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Cited by 7 •
2013
Biocompatible pH responsive sol-gel nanosensors can be incorporated into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds. The produced self-reporting scaffolds can be used for in situ monitoring of microenvironmental conditions whilst culturing cells upon the scaffold. This is beneficial as the 3D cellular construct can be monitored in real-time without disturbing the experiment.
16:03
The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
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Cited by 33 •
2011
Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included.
FAQs
Porosity does more than create space for cells: it provides physical guidance for developing neural cells and can affect how cells attach and how neurites extend through the construct. Because these features are linked to three-dimensional organization, researchers can examine how engineered architecture shapes neural growth within a structured environment.
Polymer hydrolysis gives the scaffold a temporary role during tissue development. As hydrolysis proceeds, the material gradually breaks down while new tissue forms, linking the scaffold’s physical support to changing neural organization. This degradation behavior allows researchers to investigate whether a temporary structure can support neural development over time.
Researchers can vary the scaffold’s porous architecture and degradation behavior to examine different cellular responses. These design dimensions may influence cell attachment, neurite extension, and the surrounding cellular environment. Studying them separately or together helps connect material characteristics with the organization and development of neural cells.
A neural tissue engineering study can use the scaffold as an organized three-dimensional setting for neural cells, then examine attachment, neurite extension, and changes in the local cellular environment. The architecture and degradation behavior serve as experimental variables, allowing investigators to relate material design to observed neural responses.
These scaffolds are useful when a study requires a three-dimensional context for neural development, injury modeling, or investigation of nerve repair strategies. Their physical guidance and gradual breakdown allow researchers to explore how neural cells respond to a temporary engineered environment, making the system relevant to both basic and regenerative neuroscience.
In regenerative neuroscience, the scaffold provides a designed environment in which neural cells can attach, extend neurites, and organize as the material changes over time. This combination of structural guidance and tunable degradation helps investigators study biomaterial-based approaches to nerve repair and assess how scaffold properties may influence developing neural tissue.