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SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval
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SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval

Article DOI: 10.3791/1916-v • 10:49 min • April 23rd, 2010
April 23rd, 2010 •
1, 2, 3, 1,4
1Yerkes National Primate Research Center, Emory University, 2Department of Neurology, Institute of Clinical Medicine, Tsukuba University, 3Department of Pathology, New York University School of Medicine, 4Department of Neurology, Emory University

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We describe a technique for the preparation of clarified human cortical homogenates, protein separation by SDS-PAGE, antigen retrieval and immunoblotting with an antibody to the Aβ peptide. Using this protocol, we consistently detect monomeric and multimeric Aβ in cortical tissue from humans with Alzheimer's pathology.

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SDS-PAGE/Immunoblot Aβ Multimers Human Cortical Tissue Homogenates Antigen-epitope Retrieval Anomalous Folding Polymerization β-amyloid Peptide Neurodegenerative Cascade Alzheimer's Disease Pathogenesis Self-aggregation Amyloid Fibrils Cerebral Senile Plaques Low Molecular Weight Soluble Aβ Multimers Toxic Multimers Brain Tissue Proteopathies SDS-polyacrylamide Gel Electrophoresis Immunoblotting Aβ-specific Antibodies Highly Concentrated Cortical Homogenates Small Pore-size Nitrocellulose Membranes Clarified Human Cortical Homogenates
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