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Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
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Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

Article DOI: 10.3791/4218-v • 12:04 min • August 14th, 2012
August 14th, 2012 •
1, 1,2, 2,3, 2, 1,2
1Raymond and Beverly Sackler Foundation, 2The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, 3School of Natural Sciences, Institute for Advanced Study, Princeton, New Jersey

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We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.

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Neuroendocrine Tumor Human Cell Lines Three-dimensional Model Study Therapy Incidence Malignancies Systemic Therapy Biology Therapeutic Targets Nonresectable Metastatic Neuroendocrine Tumors 3D Cell Culture Drug Screening Tumor Tissue Organization Microenvironment Multicellular Spheroids In Vitro Model Agarose-coated Plate Physiological Conditions Drug Treatments
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