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Journal / Genetics / Fare ve insan hematopoetik progenitör hücre…
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Genetics

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JoVE Journal Genetics
Fare ve insan hematopoetik progenitör hücre tarafından CRISPR/Cas9 yüksek verimli Gene bozulma
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Fare ve insan hematopoetik progenitör hücre tarafından CRISPR/Cas9 yüksek verimli Gene bozulma

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

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Bir iletişim kuralı için hızlı fare CRISPR/Cas9-aracılı gene bozulma ve insan birincil hematopoetik hücreler bu makalede açıklanan. Cas9-sgRNA ribonucleoproteins elektroporasyon ile vitro transkripsiyon ve ticari Cas9 ile oluşturulan sgRNAs ile tanıtılmaktadır. Yüksek Düzenleme verimliliği sınırlı zaman ve maliyet ile elde edilir.

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Genetik sayı: 134 CRISPR-Cas9 HSPCs hematopoetik kök ve progenitör hücre knock-out Ribonükleoprotein gen düzenleme,
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