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Neuroscience

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JoVE Journal Neuroscience
Bruk ferdigmontert plast Microfluidic sjetonger for Compartmentalizing primære Murine nerveceller
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Bruk ferdigmontert plast Microfluidic sjetonger for Compartmentalizing primære Murine nerveceller

Article DOI: 10.3791/58421-v • 10:50 min • November 2nd, 2018
November 2nd, 2018 •
1,2, 3, 3, 1,2,3
1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering, UNC, 3Xona Microfluidics, LLC

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Denne protokollen beskriver bruken av plast sjetonger for kultur og fordeler primære murine neurons. Disse brikkene er prefabrikkerte, brukervennlig og kompatibel med høy oppløsning, lever, og fluorescens bildebehandling. Denne protokollen beskriver hvordan plate rotte hippocampus nerveceller i disse brikkene og utføre fluidic isolasjon, axotomy og immunostai.

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Re-problemet 141: Microfluidic kammer Nevron kultur hippocampus neurons XonaChip microfluidic chip kultiverte nerveceller compartmentalized kammer primære nerveceller chip axon isolasjon
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