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Kontrollerad stam av 3D-hydrogeler under levande mikroskopiavbildning
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Kontrollerad stam av 3D-hydrogeler under levande mikroskopiavbildning

Article DOI: 10.3791/61671-v • 07:41 min • December 4th, 2020
December 4th, 2020 •
1, 2, 3, 3, 2, 3,4
1Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering, Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems, Tel-Aviv University

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Den presenterade metoden innebär enaxial sträckning av 3D mjuka hydrogeler inbäddade i silikongummi samtidigt som levande konfokal mikroskopi tillåts. Karakterisering av de yttre och inre hydrogelstammarna samt fiberjustering demonstreras. Enheten och protokollet som utvecklats kan bedöma cellernas reaktion på olika stamregimer.

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Bioengineering utgåva 166 Hydrogel Extracellulär matris Mekanobiologi Cellmekanik Mekanisk kraft Extern sträckning Fibröst nätverk Fiberjustering
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