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Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections (Video) | JoVE

Investigating Intestinal Barrier Breakdown in Living Organoids

Journal (Medicine)

Investigating Intestinal Barrier Breakdown in Living Organoids

Left Coronary Artery Ligation: A Surgical Murine Model of Myocardial Infarction

Journal (Medicine)

Left Coronary Artery Ligation: A Surgical Murine Model of Myocardial Infarction

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Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections (Video) | JoVE

Innovative Lung Imaging Using Agarose-Embedded Vibratome Sections

Article DOI: 10.3791/65943-v • 01:35 min • October 6th, 2023

October 6th, 2023 •

Qi Wang^1,2,3, Nicholas B. Bechet^1,2,3, Sandra Lindstedt^1,2,3,4

¹Wallenberg Centre for Molecular Medicine, Lund University, ²Department of Clinical Sciences, Lund University, ³Lund Stem Cell Centre, Lund University, ⁴Department of Cardiothoracic Surgery and Transplantation, Skåne University Hospital

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Automatiserad vibratomsnittning agarosinbäddad lungvävnad multiplexfluorescensavbildning lungvävnadsbearbetning paraffininbäddning OCT-inbäddning mikrotom kryostat precisionsskurna lungskivor primär lungvävnadsodling avancerade ljusavläsningar reproducerbar avbildning antikroppar artkompatibilitet vävnadsbearbetningsrörledning fast lungvävnad automatiserad vibratomsnittssektionering lungsektionstjocklek antigenhämtning naturlig vävnadskonservering rumsligt proteinuttryck Kvantifiering lungskada och regenerering

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