Formal Correction: Erratum: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
Posted by JoVE Editors on 3/18/2019. Citeable Link.
An erratum was issued for: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes. There were typos in the Protocol section and Table 2.
Step 3.1.1 in the Protocol was updated from:
Add 80 µl of 1 mM Pefa Bloc and 60 mg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).
to:
Add 80 µl of 1 mM Pefa Bloc and 60 µg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling).
Step 5.2 in the Protocol was updated from:
Further dilute the Control Peptide from step 5.1 (20 mM) 1:100 into the MHC II Master Mix from step 3.2.
to:
Further dilute the Control Peptide from step 5.1 (20 µM) 1:100 into the MHC II Master Mix from step 3.2.
Table 2 was updated from:
| MHC II Allele DRB1*: | 1501 |
| MHC II Stock Concentration (mg/ml) | 1.3 |
| MHC II Stock Concentration (mM) | 20 |
| Vol. MHC II Stock to Add (ml) | 14.65 |
| Vol. Reaction Buffer to Add (ml): | 2885.35 |
| MHC II Master Mix Concentration (nM) | 101 |
to:
| MHC II Allele DRB1*: | 1501 |
| MHC II Stock Concentration (mg/ml) | 1.3 |
| MHC II Stock Concentration (μM) | 20 |
| Vol. MHC II Stock to Add (μl) | 14.65 |
| Vol. Reaction Buffer to Add (μl): | 2885.35 |
| MHC II Master Mix Concentration (nM) | 101 |