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Chapters
- 00:05Title
- 01:20Binding and Capture (Day 1)
- 03:19Detection and Analysis (Days 2-3)
- 05:06Results: Binding Curves for Representative Test Peptides
- 07:29Conclusion
Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design. Here, a peptide-MHC II binding assay scaled to 384-well plates is described. This cost effective format should prove useful in the fields of protein deimmunization and vaccine design and development.
