Human Lung Model
Human Lung Model - Related Videos
10:10
A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
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Cited by 40 •
2015
Human tuberculosis infection is a complex process, which is difficult to model in vitro. Here we describe a novel 3D human lung tissue model that recapitulates the dynamics that occur during infection, including the migration of immune cells and early granuloma formation in a physiological environment.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
05:47
Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling
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Cited by 46 •
2019
Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical studies.
08:58
Development of a Neonatal Piglet Acute Lung Injury Model Recreating the Early Environment of Preterm Infant Lungs
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2025
This protocol describes the development of a neonatal piglet model of acute lung injury that models early pathogenic events occurring in the preterm lung, including sub-adequate surfactant quantity, hyperoxia, high-pressure ventilation, and inflammation, to facilitate understanding of molecular triggers of bronchopulmonary dysplasia and enhance therapeutic translation.
FAQs
An air-liquid interface exposes the lung-facing surface to air while supplying nutrients from the opposite side. This arrangement helps reproduce conditions relevant to airway and alveolar epithelial barriers more closely than fully submerged cultures. In bioengineering studies, it supports examination of barrier behavior, respiratory biology, and responses to inhaled substances under controlled experimental conditions.
These platforms provide different ways to organize lung cells and reproduce aspects of tissue architecture. Three-dimensional scaffolds support spatial organization, organoids create multicellular structures, and microfluidic devices allow tightly controlled culture environments. Their use enables researchers to investigate lung function and injury while adjusting structural or environmental conditions that are difficult to reproduce in conventional systems.
Breathing-like mechanical forces add a physical stimulus that complements cellular and structural features. Applying these forces allows researchers to study lung biology under conditions that better reflect respiratory movement than static culture alone. This is particularly relevant when examining tissue injury or evaluating whether a regenerative strategy performs under mechanically active conditions.
The model should incorporate lung-relevant cell types and features that match the question being studied. Depending on the objective, researchers may prioritize epithelial barrier function, gas-exchange processes, infection, inflammation, or tissue injury. Selecting features deliberately helps align the experimental system with a specific disease mechanism, therapeutic question, or regenerative application.
A typical workflow begins by selecting relevant lung cell types and a suitable platform, such as a three-dimensional scaffold, organoid, or microfluidic device. Researchers then establish the intended culture conditions, including an air-liquid interface or breathing-like mechanical forces when appropriate. The system can subsequently be used to examine defined biological responses or treatment effects under controlled conditions.
Engineered lung systems provide controlled platforms for evaluating how lung-relevant tissues respond to drugs or inhaled therapeutics. Their air-facing configuration can be useful when the treatment is intended for respiratory exposure, while measured changes in barrier function, inflammation, infection, or injury can connect treatment to biological outcomes. These studies help bridge experimental testing and clinically relevant lung applications.
Researchers may use these models when they need controlled investigation of human lung biology or want to reduce reliance on animal studies. The systems can isolate selected mechanisms, apply defined physical conditions, and test therapies in a human-cell-based experimental context. They therefore complement, rather than automatically replace, other approaches in respiratory research and therapeutic development.
Regenerative strategies can be evaluated in systems that combine relevant lung cells with three-dimensional structure and, when appropriate, mechanical stimulation. Researchers can examine whether an intervention supports tissue-related responses under conditions designed to reflect lung function. This provides a bridge between fundamental bioengineering experiments and clinically relevant efforts to address lung injury or restore respiratory tissue.