Omental Tissue Injection
Omental Tissue Injection - Related Videos
07:36
Intra-Omental Islet Transplantation Using h-Omental Matrix Islet filliNG (hOMING)
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Cited by 4 •
2019
Here, we present a protocol for in vivo validation of hydrogel-based cell therapy, illustrated by the example of islet transplantation. h-Omental Matrix Islet filliNG (hOMING) implantation allows implantation of a cell-hydrogel mixture between the omental layers, near to blood vessels, to maximize engraftment in a proper metabolic environment.
11:32
Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering
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Cited by 14 •
2010
Methods for preparing an injectable matrix gel from decellularized tissue and injecting it into rat myocardium in vivo are described.
10:28
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Cited by 8 •
2018
The differentiation of white and beige adipocytes from adipose tissue vascular progenitors bears potential for metabolic improvement in obesity. We describe protocols for a CD34+CD31+ endothelial cell isolation from human fat and for a subsequent in vitro expansion and differentiation into white and beige adipocytes. Several downstream applications are discussed.
02:56
Orthotopic Injection: Implanting Tissue Specific Cancer Cells into an Adult Mouse
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2023
The video demonstrates the breast cancer orthotopic inoculation technique in a mouse model. This technique produces a high tumorigenesis rate with less variability in tumor size and shape compared to subcutaneous or non-surgical injection.
03:20
Intraperitoneal Injection of Cells: A Method of Delivering Cancer Cells into the Peritoneal Adipose Tissue of Murine Model
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2023
In this video, we perform an intraperitoneal injection of ovarian cancer cell suspension in a mouse model to study the colonization of ovarian cancer cells in peritoneal adipose tissues and their subsequent...
FAQs
The omentum’s vascularization and direct tissue contact create local conditions that can affect how long a payload remains at the injection site, how it integrates with surrounding tissue, and how biologically active it becomes. Local confinement is therefore not merely a delivery feature; it provides a way to examine interactions between engineered materials or cells and a living tissue environment.
Outcomes depend on the relationship between the engineered payload and its placement within omental tissue. Payload composition, local confinement, tissue contact, and access to the omentum’s blood supply can each influence retention, integration, and biological activity. These factors help bioengineers connect properties controlled during fabrication with behavior observed after delivery into living tissue.
This approach links controlled fabrication with a physiologically relevant tissue site. Instead of examining a construct only as an engineered product, researchers can assess how it behaves when placed in contact with vascularized living tissue. That connection supports evaluation of biocompatibility, vascularization, immune responses, and therapeutic performance within the same bioengineering study.
A study first prepares the selected payload, which may include cells, biomaterials, or therapeutic agents, as an engineered construct or delivery system. A needle then places that payload within omental tissue. Researchers can subsequently evaluate its interaction with the tissue, including retention, integration, biological activity, and responses relevant to the intended therapy.
Post-injection evaluation can address several complementary outcomes: whether the material or cells remain confined, whether they integrate with surrounding tissue, and whether they retain biological activity. Bioengineering studies may also examine biocompatibility, vascularization, immune responses, and therapeutic performance. Together, these measurements indicate how the payload functions in a living tissue environment.
Researchers can use this procedure to study tissue-engineered constructs, cell-based therapies, drug delivery systems, and implantable materials. Its value is especially relevant when a project requires information about tissue contact, vascularization, immune responses, or therapeutic effects. Findings from the omental site can help evaluate regenerative strategies before they advance to later stages of development.