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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

DOI:
10.3791/57804-v

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10:28 min

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July 17, 2018

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, , , , , , ,
1Department of Physiological Sciences,Eastern Virginia Medical School

Chapters

  • 00:04Title
  • 01:01Adipose Stromal Vascular Fraction Isolation
  • 03:07Isolation of Adipose Tissue Endothelial Cells
  • 06:08Induction of Adipogenesis in Isolated Endothelial Cells
  • 07:13Results: CD31+CD34+ have Endothelial Phenotype and Display Depot- and Subject-specific Responses to Adipogenic Induction
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

The differentiation of white and beige adipocytes from adipose tissue vascular progenitors bears potential for metabolic improvement in obesity. We describe protocols for a CD34+CD31+ endothelial cell isolation from human fat and for a subsequent in vitro expansion and differentiation into white and beige adipocytes. Several downstream applications are discussed.

Tags

Keywords: CD34+CD31+ Endothelial CellsAdipogenesisObesityCell IsolationCollagenase DigestionStromal Vascular Fraction

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