Normal Human Bronchial Epithelial Cells
Normal Human Bronchial Epithelial Cells - Related Videos
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
09:12
Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
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Cited by 13 •
2016
Primary human bronchial epithelial cells are difficult to transduce. This protocol describes the production of lentiviruses and their concentration as well as the optimal culture conditions necessary to achieve highly efficient transductions in these cells throughout differentiation to a pseudostratified epithelium.
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
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Cited by 135 •
2013
Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.
11:46
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
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Cited by 29 •
2011
The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.
09:07
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Cited by 27 •
2013
This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
FAQs
Polarization organizes the cells into an airway-like epithelial layer with distinct functional surfaces. This organization allows the culture to reproduce coordinated barrier behavior and surface transport more effectively than an unstructured cell population. In bioengineering, polarized layers therefore provide a more physiologically relevant foundation for airway-on-a-chip platforms and engineered respiratory tissues.
Tight junctions connect neighboring epithelial cells and regulate how easily substances pass through the layer. Their activity makes barrier permeability an experimentally relevant feature of engineered airway models. By incorporating this junction-controlled barrier, researchers can examine airway integrity and changes associated with inflammation, infection, toxic exposures, or tissue repair in a controlled in vitro setting.
Ciliated cells contribute active surface movement that transports mucus across the epithelial layer. Including this specialized function helps engineered models represent airway clearance rather than merely reproducing a passive cellular barrier. This feature is particularly relevant when bioengineered systems are designed to study respiratory physiology, airway injury, or how environmental exposures affect epithelial surface behavior.
Their human origin and specialized airway functions can improve the biological relevance of engineered platforms. When the cells form polarized layers with tight junctions and ciliated surface activity, the resulting model captures several features of human airway biology simultaneously. This makes the system more representative for studying respiratory processes than models that reproduce fewer airway-specific characteristics.
The cells can first be expanded in culture and then differentiated under controlled conditions. Differentiation supports formation of a polarized epithelial layer containing barrier-regulating tight junctions and mucus-moving ciliated cells. This staged approach enables researchers to move from obtaining sufficient cellular material to establishing a more physiologically relevant airway model for subsequent experiments.
Researchers can incorporate these cells into airway-on-a-chip systems, engineered respiratory tissues, and other in vitro platforms. Such models support investigations of inflammation, infection, toxic exposures, and tissue repair while retaining features of human airway biology. Their use is valuable when experimental goals require a controlled engineered environment with a relevant epithelial barrier and specialized surface functions.