Centrifugation Spermatozoa Separation
Centrifugation Spermatozoa Separation - Related Videos
06:41
A Technique for Percutaneous Epididymal Spermatozoa Aspiration and Processing
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2026
Source:Li Xiaoming1, Liu Xiao1, Li Haiyang1, Cai Xiaohua1, Ou Jianping11Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University.This video demonstrates the percutaneous aspiration of epididymal spermatozoa from a patient with azoospermia, a condition characterized by the absence of spermatozoa in the semen. The procedure involves disinfecting the scrotal area, aspirating epididymal fluid using a syringe with a specialized culture solution and processing the...
09:30
Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
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Cited by 26 •
2014
Proteomic analysis of any cell type is highly dependent on both purity and pre-fractionation of the starting material in order to de-complexify the sample prior to liquid chromatography mass spectrometry (MS). By using back-flushing techniques, pure spermatozoa can be obtained from rodents. Following digestion, phosphopeptides can be enriched using TiO2.
11:42
Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
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Cited by 8 •
2017
Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.
07:07
Working with Centrifuges
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2023
Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA Earth's gravitational force is capable of separating heterogeneous mixtures. However, many systems avert this type of separation owing to the length of time usually required in such cases. Centrifugation is one of the most powerful tools for the separation of heterogeneous mixtures1. It involves the application of centripetal force for sedimentation of the heavier phase, leading to separation of...
10:24
Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
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Cited by 8 •
2018
This protocol describes a detailed methodology for detecting H2O2 localization within Solea senegalensis spermatozoa using a sensitive fluorochrome DCFH-DA for ROS, a live mitochondria stain for mitochondria, and DAPI for nuclei visualization, respectively. The protocol is designed to be performed within 2 h with either fresh or thawed spermatozoa.
FAQs
A density gradient creates layered media with different densities. During centrifugation, spermatozoa move through these layers and collect in a pellet, while seminal plasma, debris, and less dense components remain above it. This arrangement enriches the recovered fraction for subsequent washing, assessment, or preparation for fertilization procedures.
Centrifugal force drives the movement of spermatozoa and other components through the separation medium, but the recovered cells must retain viability and function. Careful control of force and processing conditions helps limit damage during isolation, producing a fraction suitable for concentration, motility, morphology assessment, or later reproductive procedures.
Particles move during spinning according to their density and size, so spermatozoa do not distribute identically to seminal plasma, debris, or other less dense components. In a layered medium, these physical differences determine how far components travel and where they accumulate, allowing the pellet to become enriched in the desired sperm fraction.
A typical workflow places semen or another biological suspension into a density-gradient protocol, applies centrifugation, and recovers the sperm-containing pellet. The pellet is then washed to remove remaining surrounding material and resuspended for analysis or use. Each stage depends on controlled processing conditions to preserve the recovered cells’ viability and function.
After washing and resuspension, the recovered sperm fraction can support measurements of concentration, motility, and morphology. These assessments characterize the separated cells rather than the original suspension alone. The resulting information can help evaluate sperm quality and determine whether the preparation is appropriate for subsequent fertilization procedures.
Centrifugation spermatozoa separation is relevant when sperm cells must be concentrated from semen or another biological suspension for semen analysis or assisted reproduction. It provides a prepared fraction that can be examined for concentration, motility, and morphology, or used in fertilization procedures after washing and resuspension.