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Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
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Developmental Biology
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JoVE Journal Developmental Biology
Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

DOI:
10.3791/55237-v

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11:42 min

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August 21, 2017

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, , , , , , ,
1Department of Animal Reproduction,Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2Department of Animal Medicine and Surgery, School of Veterinary Medicine,Universidad Complutense de Madrid, 3Department of Physiology, Faculty of Veterinary Science,University of Murcia, Campus Mare Nostrum, 4Mundomar, Benidorm, 5Veterinary Services,L’Oceanográfic, Ciudad de las Artes y las Ciencias, Junta de Murs i Vals, s/n, 46013

Chapters

  • 00:05Title
  • 01:26Dolphin Sperm Collection
  • 02:44Dolphin Sperm Evaluation and Cryopreservation
  • 04:20Heterologous In Vitro Fertilization Using Zona-intact Bovine Oocytes: Set-up
  • 07:23Heterologous In Vitro Fertilization Using Zona-intact Bovine Oocytes
  • 09:01Results: Analysis of Frozen Dolphin Spermatozoa and Dolphin-bovine Hybrid Embryos
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.

Tags

Keywords: Bottlenose DolphinSpermatozoaCryopreservationHeterologous In Vitro FertilizationSperm CollectionSperm EvaluationSperm MotilitySperm ViabilitySperm MorphologyLiquid Nitrogen VaporAssisted Reproductive TechnologiesMale Pronuclear FormationHybrid Embryo Cleavage

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