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JoVE Journal
Biology

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Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
 

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

Article DOI: 10.3791/51546
December 30th, 2014

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Summary December 30th, 2014

Proteomic analysis of any cell type is highly dependent on both purity and pre-fractionation of the starting material in order to de-complexify the sample prior to liquid chromatography mass spectrometry (MS). By using back-flushing techniques, pure spermatozoa can be obtained from rodents. Following digestion, phosphopeptides can be enriched using TiO2.

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