Genome Organization
Genome Organization - Related Videos
08:31
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
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Cited by 2 •
2013
Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Cited by 45 •
2018
Utilizing a preassembled Cas9 ribonucleoprotein complex (RNP) is a powerful method for precise, efficient genome editing. Here, we highlight its utility across a broad range of cells and organisms, including primary human cells and both classic and emerging model organisms.
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
09:30
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
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Cited by 11 •
2018
This protocol provides researchers with a new tool to monitor the fidelity of transcription in multiple model organisms.
12:04
Mouse Genome Engineering Using Designer Nucleases
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Cited by 14 •
2014
Designer nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can be used to modify the genome of mouse preimplantation embryos by triggering both the nonhomologous end joining (NHEJ) and homologous recombination (HR) pathways. These advances enable the rapid generation of mice with precise genetic modifications.
FAQs
Histones provide the proteins around which DNA winds to form nucleosomes. This packaging step creates the initial structural level from which chromatin develops. Rather than treating DNA as an unstructured sequence, researchers can examine nucleosome formation as part of the arrangement that helps genetic material fit inside the nucleus while preserving access for cellular regulation.
These higher-order features bring particular parts of the genome into defined spatial relationships. Such positioning can influence whether genes interact with regulatory elements, linking three-dimensional arrangement to gene expression. This makes genome organization relevant beyond DNA packaging: spatial relationships may help explain how cells control genes and why cellular behavior differs across biological contexts.
Genome organization matters because the placement and folding of DNA are connected to regulation rather than serving only a packaging function. Environmental signals can be studied in relation to how cells control genes and alter their responses. This connection helps biology link nuclear structure with changing cellular states without treating gene regulation as an isolated sequence-level process.
Three-dimensional genome technologies help researchers investigate DNA structure in relation to cellular function. Their value lies in connecting spatial features, such as loops, domains, and chromosome territories, with interactions between genes and regulatory elements. This approach can explain how cells control gene expression while accounting for the arrangement of genetic material inside the nucleus.
These studies provide a structural perspective on chromosome abnormalities by examining how chromosome arrangement relates to genome function. The same framework supports investigation of genetic disease because researchers can consider organization alongside gene regulation and cellular behavior. This produces a broader biological view that connects chromosome structure with potential cellular consequences rather than focusing on DNA sequence alone.
Cell specialization depends on controlled gene expression, and genome organization provides a structural context for that control. During development, the arrangement of DNA and its regulatory relationships can be studied alongside the emergence of distinct cell types. This makes the topic useful for connecting nuclear architecture with developmental processes and explaining how cells acquire different biological roles.