Phagocytic Macrophages
Phagocytic Macrophages - Related Videos
03:06
The Effect of Aspirin and Ibuprofen on the Phagocytic Ability of Macrophages
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2025
Source:Adepemi O. Ogundeji1, Nozethu Mjokane1, Maphori Maliehe1, Olihile M. Sebolai11Department of Microbiology and Biochemistry, University of the Free State.This video showcases a method to assess the impact of aspirin and ibuprofen, at their minimum inhibitory concentrations, on macrophage phagocytosis using pH-sensitive fluorescent dye-stained fungal cells. The drugs inhibit cyclooxygenase-2, decreasing prostaglandin formation and boosting macrophage phagocytosis, as observed through...
03:45
Measuring Macrophage Phagocytic Efficiency with Primaquine and Photodynamic Therapy
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2025
Source:Uju L. Madu1, Maphori Maliehe1, Nozethu Mjokane1, Olihile M. Sebolai11Department of Microbiology and Biochemistry, University of the Free State.The video demonstrates evaluating photodynamic treatment's impact on murine macrophage phagocytic efficiency with primaquine. Treated, infected macrophages generate reactive oxygen species, leading to selective fungal death, evident by reduced colonies on the plate.
09:25
Purification of Pathogen Vacuoles from Legionella-infected Phagocytes
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Cited by 5 •
2012
This article describes a method for the isolation and purification of intact Legionella-containing vacuoles (LCVs) from amoeba and macrophages. The two-step protocol comprises LCV enrichment by immuno-magnetic separation using an antibody against a bacterial LCV marker and further purification by density gradient centrifugation.
09:05
Determining the Phagocytic Activity of Clinical Antibody Samples
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Cited by 33 •
2011
We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.
04:03
An In Vitro Assay to Measure Antibody-Dependent Cellular Phagocytosis by Phagocytes
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2025
This video demonstrates an assay to measure the antibody-dependent cellular phagocytosis (ADCP) by breast milk phagocytes. Fluorescently labeled microspheres conjugated to an antigen-antibody complex, are combined with a cell suspension isolated from breast milk to induce antibody-dependent cellular phagocytosis by the phagocytic leukocytes. Upon labeling the cells with antibodies against common leukocyte antigen (CD45), flow cytometry is used to measure the fluorescence intensity of...
FAQs
Surface receptors enable phagocytic macrophages to identify pathogens, dead cells, and cellular debris before engulfment begins. Recognition helps the cell distinguish material that requires removal or response from surrounding tissue. After detection, extensions of the plasma membrane surround the target, linking receptor-mediated identification to formation of an internal phagosome.
The phagosome proceeds toward fusion with lysosomes, which contain enzymes and reactive molecules capable of breaking down its contents. This intracellular processing converts engulfed material into components the cell can handle while removing potentially harmful substances. The sequence is central to both pathogen control and the clearance functions that support tissue homeostasis.
Macrophages respond not only to infectious pathogens but also to dead cells and cellular debris. Engulfing these different targets gives the cells both a defensive and a maintenance role: they help protect tissue during infection while also clearing unwanted material. This combination explains their importance in preserving local tissue condition and biological balance.
Phagocytic macrophages provide a functional link between immediate immune defense and ongoing tissue maintenance. Their activity contributes to the innate immune response during infection, while removal of dead cells and debris supports homeostasis. Studying these cells therefore helps biology researchers examine how immune activity and tissue stability are coordinated rather than treated as separate processes.
Their activity influences wound healing and tissue remodeling in addition to pathogen removal. By processing dead cells and cellular debris, macrophages can affect the material present in damaged tissue, while their broader immune activity contributes to the local response. These functions make them relevant to research on how tissues respond to injury and undergo structural change.
Macrophage activity is connected not only with protective immunity but also with disease processes, including chronic inflammation and tumor development. This makes them important subjects for investigating how an immune response may relate to persistent tissue changes or abnormal growth. Research can examine their engulfment activity alongside the wider effects of macrophage behavior in diseased tissues.