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JoVE Journal
Immunology and Infection

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Determining the Phagocytic Activity of Clinical Antibody Samples
 

Determining the Phagocytic Activity of Clinical Antibody Samples

Article doi: 10.3791/3588
November 30th, 2011

Summary November 30th, 2011

We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.

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