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Determining the Phagocytic Activity of Clinical Antibody Samples
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Chapters
- 00:05Title
- 02:01Preparing Biotinylated Antigen
- 02:58Preparing Antigen Saturated Beads
- 04:08Preparing Antibody Samples and Plating the Experiment
- 05:44Flow Cytometric Analysis
- 06:29Antigen-saturated Beads and Phagocytosed in an Antibody-dependent Manner
- 08:24Conclusion
We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.
Tags
Phagocytic ActivityClinical Antibody SamplesFc ReceptorsClearancePathology Of DiseaseVariable DomainsInnate Immune CellsRecombinant Monoclonal Antibody TherapyNatural InfectionVaccinationIgG SubclassGlycosylationFunctional DifferencesAntigen-specific AntibodiesDisease StateSusceptibility To InfectionProgressionClinical OutcomeEffector FunctionEnhance InfectionFc Receptor-driven PhagocytosisImmune ComplexesTh1/Th2 Polarization Assay
