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Immunology and Infection

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JoVE Journal Immunology and Infection
Determining the Phagocytic Activity of Clinical Antibody Samples
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Determining the Phagocytic Activity of Clinical Antibody Samples

Article DOI: 10.3791/3588 • 09:06 min
November 30th, 2011 •
1, 1, 1, 1, 1, 2
1Massachusetts General Hospital, Ragon Institute of MGH, MIT, and Harvard, 2Thayer School of Engineering, Dartmouth College

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November 30th, 2011

We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.

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